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MPG Antibody

Images

 
Orthogonal Strategies: Western Blot: MPG Antibody [NBP1-82787] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: MPG Antibody [NBP1-82787] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MPG Antibody [NBP1-82787] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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MPG Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MPG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
WB, ICC/IF reported in scientific literature (PMID: 23290262). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MPG Protein (NBP1-82787PEP)
Reviewed Applications
Read 2 Reviews rated 3
using
NBP1-82787 in the following applications:

Publications
Read Publications using
NBP1-82787 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (84%). Human reactivity reported in scientific literature (PMID: 23290262).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MPG Antibody

  • 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on16p
  • 3-alkyladenine DNA glycosylase
  • 3-methyladenine DNA glycosidase
  • AAG
  • ADPG
  • anpg
  • APNG
  • CRA36.1 (3-methyl-adenine DNA glycosylase)
  • CRA36.1
  • DNA-3-methyladenine glycosylase
  • EC 3.2.2.21
  • MDGalkyladenine DNA glycosylase
  • Mid1
  • N-methylpurine-DNA glycosylase, MPG
  • N-methylpurine-DNA glycosylasePIG16
  • PIG11
  • proliferation-inducing protein 11
  • proliferation-inducing protein 16

Background

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC

Publications for MPG Antibody (NBP1-82787)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Filter By Species
Human
(1)
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Reviews for MPG Antibody (NBP1-82787) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-82787:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry MPG NBP1-82787
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3
reviewed by:
Verified Customer
ICC Human 11/16/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedHEK293
SpeciesHuman
LotA96602

Comments

Comments4ug/mL overnight at 4C in 1X PBS/1% BSA/0.3% Triton™ X-100. Nuclear signal not as strong and seemed more diffuse when compared to staining done with another MPG antibody done at the same time.
Western Blot MPG NBP1-82787
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3
reviewed by:
Verified Customer
WB Human 09/20/2018
View

Summary

ApplicationWestern Blot
Sample TestedLymphocytes
SpeciesHuman
LotA96602

Comments

Comments30ug of Lymphocyte whole cell lysate. Antibody used at 0.4ug/mL in 5% milk-TBS/T. 5 minute exposure.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MPG Antibody (NBP1-82787) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for MPG Antibody (NBP1-82787)

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Blogs on MPG

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Contact Information

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Calculators

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Recent Reviews

3
5
0
4
0
3
2
2
0
1
0

Verified Customer
11/16/2018
Application: ICC
Species: Human

Verified Customer
09/20/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol MPG