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Mouse TNF-alpha DuoSet ELISA, 15 Plate

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Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Mouse TNF-alpha DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Tnf

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DY410 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse TNF-alpha DuoSet ELISA, 15 Plate

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer (5-11).   
Mouse TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (12). Within the ECD, mouse TNF-alpha shares 95% aa identity with rat, and 80% aa identity with canine, equine, feline, human, rabbit, and porcine TNF-alpha . It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (13). Cell surface TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling following ligation by soluble TNF RI (14, 15). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer containing the TNF-alpha extracellular domain (16-18).    
TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (19, 20) and the hematopoietic cell-restricted 78-80 kDa TNF RII (21, 22), both of which are also expressed as homotrimers (1, 23). Both type I and type II receptors bind TNF-alpha with comparable affinity and can promote NFkB activation (24-27). Only TNF RI, however, contains a cytoplasmic death domain which triggers the activation of apoptosis (3, 28). Soluble forms of both types of receptors are released into human serum and urine, and can neutralize the biological activity of TNF (29-31).

Publications for TNF-alpha (DY410)(705)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Gerbil, Transgenic Mouse.

We have publications tested in 2 applications: ELISA, ELISA Capture.


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ELISA
(1)
ELISA Capture
(1)
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Filter By Species
Human
(2)
Mouse
(699)
Rat
(3)
Canine
(1)
Gerbil
(1)
Transgenic Mouse
(6)
All Species
Showing Publications 1 - 10 of 705. Show All 705 Publications.
Publications using DY410 Applications Species
Nakajima, S;Demers, G;Machuca-Parra, AI;Pour, ZD;Bairamian, D;Bouyakdan, K;Fisette, A;Kabahizi, A;Robb, J;Rodaros, D;Laurent, C;Ferreira, G;Arbour, N;Alquier, T;Fulton, S; Central activation of the fatty acid sensor GPR120 suppresses microglia reactivity and alleviates sickness- and anxiety-like behaviors Journal of neuroinflammation 2023-12-19 [PMID: 38111048] (Mouse) Mouse
Zhu, W;Han, L;Wu, Y;Tong, L;He, L;Wang, Q;Yan, Y;Pan, T;Shen, J;Song, Y;Shen, Y;Zhu, Q;Zhou, J; Keratin 15 protects against cigarette smoke-induced epithelial mesenchymal transformation by MMP-9 Respiratory research 2023-11-25 [PMID: 38007424] (Transgenic Mouse, Mouse) Transgenic Mouse, Mouse
Chuang, WH;Pislyagin, E;Lin, LY;Menchinskaya, E;Chernikov, O;Kozhemyako, V;Gorpenchenko, T;Manzhulo, I;Chaikina, E;Agafonova, I;Silchenko, A;Avilov, S;Stonik, V;Tzou, SC;Aminin, D;Wang, YM; Holothurian triterpene glycoside cucumarioside A2-2 induces macrophages activation and polarization in cancer immunotherapy Cancer cell international 2023-11-24 [PMID: 38001420] (Mouse) Mouse
Lopez-Rodriguez, AB;Murray, CL;Kealy, J;Towns, C;Roche, A;Nazmi, A;Doran, M;Lowry, JP;Cunningham, C; Hyperthermia elevates brain temperature and improves behavioural signs in animal models of autism spectrum disorder Molecular autism 2023-11-15 [PMID: 37968722] (Mouse) Mouse
Pereira, RDD;Rabelo, RAN;Oliveira, NFM;Porto, SLT;Andrade, ACDSP;Queiroz-Junior, CM;Barbosa, CLN;de Souza-Costa, LP;Santos, FRDS;Oliveira, FBR;da Silva, BLV;Umezu, HL;Ferreira, R;da Silva, GSF;Cruz, JS;Teixeira, MM;Costa, VV;Machado, FS; A 5-Lipoxygenase Inhibitor, Zileuton, Modulates Host Immune Responses and Improves Lung Function in a Model of Severe Acute Respiratory Syndrome (SARS) Induced by Betacoronavirus Viruses 2023-10-04 [PMID: 37896826] (Mouse) Mouse
Leinardi, R;Petriglieri, JR;Pochet, A;Yakoub, Y;Lelong, M;Lescoat, A;Turci, F;Lecureur, V;Huaux, F; Distinct Pro-Inflammatory Mechanisms Elicited by Short and Long Amosite Asbestos Fibers in Macrophages International journal of molecular sciences 2023-10-13 [PMID: 37894824] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
Huang, HC;Lee, PN;Huang, WC;Yang, HY; Partial Replacement of Diet with Dehulled Adlay Ameliorates Hepatic Steatosis, Inflammation, Oxidative Stress, and Gut Dysbiosis in Rats with Nonalcoholic Fatty Liver Disease Nutrients 2023-10-16 [PMID: 37892450] (Rat) Rat
Firth, W;Robb, JL;Stewart, D;Pye, KR;Bamford, R;Oguro-Ando, A;Beall, C;Ellacott, KL; Regulation of astrocyte metabolism by mitochondrial translocator protein 18kDa bioRxiv : the preprint server for biology 2023-10-02 [PMID: 37873215] (Human) Human
Uemura, M;Maeshige, N;Yamaguchi, A;Ma, X;Matsuda, M;Nishimura, Y;Hasunuma, T;Inoue, T;Yan, J;Wang, J;Kondo, H;Fujino, H; Electrical stimulation facilitates NADPH production in pentose phosphate pathway and exerts an anti-inflammatory effect in macrophages Scientific reports 2023-10-19 [PMID: 37857669] (Mouse) Mouse
Russo, S;Kwiatkowski, M;Wolters, JC;Gerding, A;Hermans, J;Govorukhina, N;Bischoff, R;Melgert, BN; Effects of lysine deacetylase inhibitor treatment on LPS responses of alveolar-like macrophages Journal of leukocyte biology 2023-10-09 [PMID: 37811856] (Mouse) Mouse
Show All 705 Publications.

Reviews for TNF-alpha (DY410) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF-alpha (DY410). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.
Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.
Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.
Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.
You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.
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Bioinformatics

Gene Symbol Tnf