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Mouse IL-17 Quantikine ELISA Kit, 2 Plate

 (123 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Catalog# & Conjugate Size Price
HRP
PM1700
HRP
SM1700
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Mouse IL-17 Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse IL-17 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-17 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-17 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IL-17 accurately. Results obtained using natural mouse IL-17 sh...owed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-17.
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Specificity
Natural and recombinant mouse IL-17
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il17a

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
M1700 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-17 Quantikine ELISA Kit, 2 Plate

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Mouse Interleukin 17 (IL-17; also known as IL-17A and CTLA-8) is a 21 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines containing a cysteine-knot fold (1-3). Its sequence was originally isolated from an activated hybridoma created from the fusion of a mouse cytotoxic and rat T cell lymphoma cell line (2-5). It is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 15 kDa, 133 aa mature segment (5). In both mouse and human, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 35-38 kDa homodimer, and a 45-48 kDa heterodimer with IL-17F (6, 7). Mature mouse IL-17A is 61% and 89% aa identical to human and rat IL-17A, respectively (4, 5, 8). While rodent and human mature sequences show modest aa sequence identity, human IL-17 is active on both mouse and rat cells (5, 9). Cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27-gamma δ T cells, CD1+NK1.1- iNKT cells and CD3- CD4+ LTi-like cells (3, 5, 6, 10-12). 
A high affinity receptor for mouse IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 13). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (14, 15). Both receptors are needed for IL-17A and IL-17A/F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 16). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (3, 10, 17, 18). Its induction was initially described to be the result of antigen stimulation of dentritic cells, resulting in IL-23 secretion. In a TCR-independent event, IL-23 induces T cell production of IL-17 (3). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and SCF (membrane form), an effect that increases neutrophil differentiation and activation. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating neutrophil numbers (17). In the tissues, IL-17 seems to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha , IL-1 beta and IL-6 production (19). TNF-alpha and IL-1 beta then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (20). IL-17 further contributes to neutrophil influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (3, 9). IL-17 effects are not limited to neutrophils. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (18).

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Mouse IL-17
Quantikine ELISA Kit, ...
M1700
Mouse IL-17 Quantikine ELISA Kit, 2 Plate
Species: Mu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-17/IL-17A (M1700)(123)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(1)
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Human
(2)
Mouse
(119)
Rat
(1)
Transgenic Mouse
(3)
All Species
Showing Publications 1 - 10 of 123. Show All 123 Publications.
Publications using M1700 Applications Species
Douglas, A;Stevens, B;Rendas, M;Kane, H;Lynch, E;Kunkemoeller, B;Wessendorf-Rodriguez, K;Day, EA;Sutton, C;Brennan, M;O'Brien, K;Kohlgruber, AC;Prendeville, H;Garza, AE;O'Neill, LAJ;Mills, KHG;Metallo, CM;Veiga-Fernandes, H;Lynch, L; Rhythmic IL-17 production by ?? T cells maintains adipose de novo lipogenesis Nature 2024-10-30 [PMID: 39478228] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
Gu, L;Yin, Y;Liu, M;Yu, L; Acacetin protects against acute lung injury by upregulating SIRT1/ NF-?B pathway Heliyon 2024-09-15 [PMID: 39296097] (Human, Mouse) Human, Mouse
Kim, H;Choi, J;Seo, J;Lim, H;Kang, SK; CKD-497 inhibits NF-kB signaling and ameliorates inflammation and pulmonary fibrosis in ovalbumin-induced asthma and particulate matter-induced airway inflammatory diseases Frontiers in pharmacology 2024-08-07 [PMID: 39170711] (Mouse) Mouse
Abboud, E;Chrayteh, D;Boussetta, N;Dalle, H;Malerba, M;Wu, TD;Le Gall, M;Reelfs, O;Pourzand, C;Mellett, M;Assan, F;Bachelez, H;Poupon, J;Aractingi, S;Vaulont, S;Sohier, P;Oules, B;Karim, Z;Peyssonnaux, C; Skin hepcidin initiates psoriasiform skin inflammation via Fe-driven hyperproliferation and neutrophil recruitment Nature communications 2024-08-07 [PMID: 39112467] (Transgenic Mouse) Transgenic Mouse
Xu, D;Ren, L;Zhang, W;Wu, S;Yu, M;He, X;Wei, Z; Therapeutic effects and mechanisms of fecal microbiota transplantation on EAE partly through HPA axis-mediated neuroendocrine regulation Heliyon 2024-06-30 [PMID: 39021924] (Mouse) Mouse
Jiang, Y;Gruszka, D;Zeng, C;Swindell, WR;Gaskill, C;Sorensen, C;Brown, W;Gangwar, RS;Tsoi, LC;Webster, J;Sigurdardottir, SL;Sarkar, MK;Uppala, R;Kidder, A;Xing, X;Plazyo, O;Xing, E;Billi, AC;Maverakis, E;Kahlenberg, JM;Gudjonsson, J;Ward, NL; Suppression of TCF4 promotes a ZC3H12A-mediated self-sustaining inflammatory feedback cycle involving IL-17RA/IL-17RE epidermal signaling JCI insight 2024-03-12 [PMID: 38470486] (Transgenic Mouse) Transgenic Mouse
Jung, SH;Chung, KS;Na, CS;Ahn, HS;Shin, YK;Lee, KT; Ethanol Extracts from the Aerial Parts of Inula japonica and Potentilla chinensis Alleviate Airway Inflammation in Mice That Inhaled Particulate Matter 10 and Diesel Particulate Matter Nutrients 2023-10-29 [PMID: 37960252] (Mouse) Mouse
Zhang, B;Lai, RC;Sim, WK;Lim, SK; Therapeutic Efficacy of Mesenchymal Stem/Stromal Cell Small Extracellular Vesicles in Alleviating Arthritic Progression by Restoring Macrophage Balance Biomolecules 2023-10-10 [PMID: 37892183] (Mouse) Mouse
Fan, W;Wang, X;Zeng, S;Li, N;Wang, G;Li, R;He, S;Li, W;Huang, J;Li, X;Liu, J;Hou, S; Global lactylome reveals lactylation-dependent mechanisms underlying TH17 differentiation in experimental autoimmune uveitis Science advances 2023-10-20 [PMID: 37851814] (Mouse) Mouse
Ando, M;Takahashi, M;Mizuno-Kamiya, M;Morimoto-Ito, H;Ikeno, K;Ueno, K;Takayama, E;Kawaki, H;Nakamura, G;Muramatsu, Y;Fujita, H;Kondoh, N; Enhanced production of IL-2 from anti-CD3 antibody-stimulated mouse spleen cells by caffeic acid phenethyl ester, a major component of Chinese propolis Journal of oral biosciences 2023-08-16 [PMID: 37595745] (Mouse) Mouse
Show All 123 Publications.

Reviews for IL-17/IL-17A (M1700) (0)

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Product General Protocols

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FAQs for IL-17/IL-17A (M1700). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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Bioinformatics

Gene Symbol Il17a