Home » CX3CL1/Fractalkine » CX3CL1/Fractalkine ELISA Kits » Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
Novus Biologicals products are now on bio-techne.com

Mouse CX3CL1/Fractalkine Quantikine ELISA Kit

 (11 Publications)
 See All Related
Submit a Review

Images

 

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Mouse CX3CL1/Fractalkine Quantikine ELISA Kit Summary

Background
The Quantikine Mouse Fractalkine Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Fractalkine in cell culture supernates, tissue homogenates, mouse serum, and plasma. It contains E. coli-expressed recombinant mouse Fractalkine chemokine domain and antibodies raised against the recombinant protein. Results obtained for naturally occurring soluble mouse Fractalkin...e containing the chemokine domain and the mucin stalk domain showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Fractalkine.
Show More
Specificity
Natural and recombinant mouse Fractalkine (chemokine and extracellular domains)
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Cx3cl1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MCX310.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CX3CL1/Fractalkine Quantikine ELISA Kit

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Customers Who Viewed This Item Also Viewed...

CX3CR1 Antibody
[Unconjugated]
AF5825
Flow Cytometry CX3CR1 Antibody [Unconjugated]
Western Blot CX3CR1 Antibody [Unconjugated]
(1 Review)
(15 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(809 Publications)
Species: Hu
Applications: BA
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(253 Publications)
Species: Hu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
Neurturin
1297-NE/CF
Neurturin
(9 Publications)
Species: Hu
Applications: Bind, BA
GDNF [Unconjugated]
212-GD
Bioactivity GDNF [Unconjugated]
SDS-PAGE GDNF [Unconjugated]
SEC-MALS GDNF [Unconjugated]
(213 Publications)
Species: Hu
Applications: Bind, BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(641 Publications)
Species: Mu
Applications: ELISA
CD35 Antibody (E11)
NBP2-29460
Immunohistochemistry-Paraffin CD35 Antibody (E11)
(6 Publications)
Species: Pm, Pm-Cm, Hu, Pm, RM
Applications: Flow, ICC/IF, IHC, IHC-P, ISH
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(152 Publications)
Species: Hu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(450 Publications)
Species: Hu
Applications: BA
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(182 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
CXCL12/SDF-1 alpha
[Unconjugated]
350-NS
SDS-PAGE CXCL12/SDF-1 alpha [Unconjugated]
Bioactivity CXCL12/SDF-1 alpha [Unconjugated]
(182 Publications)
Species: Fe, Hu, RM
Applications: BA, BA
CCL22/MDC [HRP]
DMD00
N/A CCL22/MDC [HRP]
N/A CCL22/MDC [HRP]
(31 Publications)
Species: Hu
Applications: ELISA
CCL17/TARC [HRP]
MCC170
N/A CCL17/TARC [HRP]
N/A CCL17/TARC [HRP]
(45 Publications)
Species: Mu
Applications: ELISA
Ret Antibody
[Unconjugated]
AF482
Immunohistochemistry Ret Antibody [Unconjugated]
Western Blot Ret Antibody [Unconjugated]
Immunohistochemistry Ret Antibody [Unconjugated]
(40 Publications)
Species: Mu
Applications: IHC, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(295 Publications)
Species: Mu
Applications: ELISA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
Mouse
CX3CL1/Fractalkine Qu ...
MCX310
Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
Species: Mu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CX3CL1/Fractalkine (MCX310)(11)

We have publications tested in 2 confirmed species: Mouse, Rat.


Filter By Application
All Applications
Filter By Species
Mouse
(10)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MCX310 Applications Species
K Inoue, H Morimoto, M Ohgidani, T Ueki Modulation of inflammatory responses by fractalkine signaling in microglia PLoS ONE, 2021-05-21;16(5):e0252118. 2021-05-21 [PMID: 34019594] (Mouse) Mouse
A Helmke, AM Hüsing, S Gaedcke, N Brauns, MS Balzer, M Reinhardt, M Hiss, N Shushakova, D de Luca, I Prinz, H Haller, S von Vietin Peritoneal dialysate-range hypertonic glucose promotes T cell IL-17 production that induces mesothelial inflammation Eur. J. Immunol., 2020-09-30;0(0):. 2020-09-30 [PMID: 32926407] (Mouse) Mouse
R Fu, H Guo, S Janga, M Choi, W Klinngam, MC Edman, SF Hamm-Alvar Cathepsin S activation contributes to elevated CX3CL1 (fractalkine) levels in tears of a Sj�gren's syndrome murine model Sci Rep, 2020-01-29;10(1):1455. 2020-01-29 [PMID: 31996771] (Mouse) Mouse
MD Dorfman, JE Krull, JD Douglass, R Fasnacht, F Lara-Lince, TH Meek, X Shi, V Damian, HT Nguyen, ME Matsen, GJ Morton, JP Thaler Sex differences in microglial CX3CR1 signalling determine obesity susceptibility in mice Nat Commun, 2017-02-22;8(0):14556. 2017-02-22 [PMID: 28223698] (Mouse) Mouse
Intravenous hMSCs Ameliorate Acute Pancreatitis in Mice via Secretion of Tumor Necrosis Factor-? Stimulated Gene/Protein 6 Sci Rep, 2016-12-05;6(0):38438. 2016-12-05 [PMID: 27917949] (Mouse) Mouse
Reed , Johanna, Stone , Matthew, Beadnell , Thomas C, Ryu , Yungil, Griffin , Timothy, Schwertfeger , Kathryn Fibroblast growth factor receptor 1 activation in mammary tumor cells promotes macrophage recruitment in a CX3CL1-dependent manner. PLoS ONE, 2012-09-24;7(9):e45877. 2012-09-24 [PMID: 23029290] (Mouse) Mouse
Tighe RM, Li Z, Potts EN, Frush S, Liu N, Gunn MD, Foster WM, Noble PW, Hollingsworth JW Ozone inhalation promotes CX3CR1-dependent maturation of resident lung macrophages that limit oxidative stress and inflammation. J. Immunol., 2011-09-19;187(9):4800-8. 2011-09-19 [PMID: 21930959] (Mouse) Mouse
Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis. J. Immunol., 2008-09-15;181(6):4208-18. 2008-09-15 [PMID: 18768878] (Mouse) Mouse
Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis. Am. J. Pathol., 2008-04-10;172(5):1209-21. 2008-04-10 [PMID: 18403600] (Mouse) Mouse
Ren T, Chen Q, Tian Z, Wei H Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice. Biochem. Biophys. Res. Commun., 2007-10-29;364(4):978-84. 2007-10-29 [PMID: 17971299] (Mouse) Mouse
Show All 11 Publications.

Reviews for CX3CL1/Fractalkine (MCX310) (0)

There are no reviews for CX3CL1/Fractalkine (MCX310). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CX3CL1/Fractalkine (MCX310) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CX3CL1/Fractalkine Products

Blogs on CX3CL1/Fractalkine

There are no specific blogs for CX3CL1/Fractalkine, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF365). The ND50 is typically 0.75-4.5 µg/mL.
CX3CL1/Fractalkine Antibody [Unconjugated]
AF365

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Mouse CX3CL1/Fractalkine Quantikine ELISA Kit and receive a gift card or discount.


Bioinformatics

Gene Symbol Cx3cl1