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MMP-9 Antibody (4H3) [Unconjugated]

Images

 
Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 5 ng/mL PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-9 Monoclonal Antibody ...read more
Effects of IGF-1 or/and PDGF-bb on IL-1 beta -induced NF-kappa B-dependent pro-inflammatory, pro-apoptotic and matrix degrading gene products in chondrocytes.To determine whether IGF-1 or/and PDGF-bbexert effects on ...read more
Genetic Strategies: MMP-9 positively regulates roundness and MLC2 activity(a) Cell morphology (roundness) of A375M2 cells on top of bovine collagen I after MMP-9 knockdown (siMMP-9). Dots represent single cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, CyTOF-ready
Clone
4H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

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Catalog# & Formulation Size Price
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MMP-9 Antibody (4H3) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Specificity
Detects human MMP-9 in Western blots. In Western blots, reactivity with the pro (92 kDa), active (82 kDa), and C-terminal truncated (65 kDa) forms of recombinant human (rh) MMP-9 is observed. Also in Western blots, 20% cross-reactivity with rhMMP-2, 5% cross‑reactivity with rhMMP-1, and no cross-reactivity with rhMMP-3, -7, -8, -10, -12, or -13 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Immunohistochemistry 25-100 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB911 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-9 Antibody (4H3) [Unconjugated]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-9 Antibody (MAB911)(26)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 8 applications: Cell-based ELISA, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Luminex Development, Western Blot.


Filter By Application
Cell-based ELISA
(1)
ICC
(2)
IHC
(1)
IHC-Fr
(2)
IHC-P
(3)
Immunoprecipitation
(1)
Luminex Development
(1)
Western Blot
(9)
All Applications
Filter By Species
Human
(16)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using MAB911 Applications Species
Valeria Fernández Vallone, Francisco Raúl Borzone, Leandro Marcelo Martinez, María Belén Giorello, Hosoon Choi, Federico Dimase et al. Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients Frontiers in Oncology 2023-02-20 [PMID: 36890825]
L Mlakar, SM Garrett, T Watanabe, M Sanderson, T Nishimoto, J Heywood, KL Helke, JM Pilewski, EL Herzog, C Feghali-Bo Ameliorating Fibrosis in Murine and Human Tissues with END55, an Endostatin-Derived Fusion Protein Made in Plants Biomedicines, 2022-11-09;10(11):. 2022-11-09 [PMID: 36359382] (Western Blot, Human) Western Blot Human
A Brockmuell, AL Mueller, P Shayan, M Shakibaei beta1-Integrin plays a major role in resveratrol-mediated anti-invasion effects in the CRC microenvironment Frontiers in Pharmacology, 2022-09-02;13(0):978625. 9/2/2022 [PMID: 36120305]
M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker Cancers, 2022-07-13;14(14):. 2022-07-13 [PMID: 35884455] (IHC, Human) IHC Human
Gemma Sullivan, Paola Galdi, Nis Borbye-Lorenzen, David Q. Stoye, Gillian J. Lamb, Margaret J. Evans et al. Preterm Birth Is Associated With Immune Dysregulation Which Persists in Infants Exposed to Histologic Chorioamnionitis Frontiers in Immunology 2021-08-27 [PMID: 34512648]
TY Hou, SB Wu, HC Kau, CC Tsai JNK and p38 Inhibitors Prevent Transforming Growth Factor-&beta1-Induced Myofibroblast Transdifferentiation in Human Graves' Orbital Fibroblasts International Journal of Molecular Sciences, 2021-03-14;22(6):. 2021-03-14 [PMID: 33799469] (Western Blot, Human) Western Blot Human
Sinead M. Walsh, Julie C. Worrell, Aurelie Fabre, Boris Hinz, Rosemary Kane, Michael P. Keane Novel differences in gene expression and functional capabilities of myofibroblast populations in idiopathic pulmonary fibrosis American Journal of Physiology-Lung Cellular and Molecular Physiology 2018-11-01 [PMID: 30091381]
Aline L. Cortes, Sabrina R. Gonsalez, Lilimar S. Rioja, Simone S.C. Oliveira, André L.S. Santos, Minolfa C. Prieto et al. Protective outcomes of low-dose doxycycline on renal function of Wistar rats subjected to acute ischemia/reperfusion injury Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2018-01-01 [PMID: 28987762]
Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V Diverse matrix metalloproteinase functions regulate cancer amoeboid migration. Nat Commun, 2014-06-25;5(0):4255. 2014-06-25 [PMID: 24963846] (ICC, IHC-P, Western Blot, Human) ICC, IHC-P, Western Blot Human
Sukhwinder Singh Sohal, Amir Soltani, David Reid, Chris Ward, Karen E Wills, H Konrad Muller et al. A randomized controlled trial of inhaled corticosteroids (ICS) on markers of epithelial–mesenchymal transition (EMT) in large airway samples in COPD: an exploratory proof of concept study International Journal of Chronic Obstructive Pulmonary Disease 2014-06-13 [PMID: 24920891]
Show All 26 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (MAB911). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP9