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MMP-9 Antibody (4A3)

Images

 
Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times.
Immunohistochemistry: MMP-9 Antibody (4A3) [NBP2-13173] - MMP-9 (green) was detected in murine brain (cortex) using MMP-9-DyLight 488 (4A3) with a concentration of 1:20 in PBS for 2 hours. MMP9-Signals are overlaped ...read more
Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Identification of gelatinases by Western blot analysis. A pool of sera from 10 patients with inactive RR-MS was used. For 2-DZ, pooled sera were concentrated 10-fold and ...read more
Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 1-DZ analysis and identification of gelatinases by Western blot. 1.5 ul of serum from healthy control (HC) or from a patient with inactive RR-MS were loaded on a 7.5% ...read more
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled ...read more
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary ...read more
Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 2-DZ analysis & identification of MMP-9 by Western blot. Left panels (A & B): 2-D zymography (2-DZ) of serum samples from healthy control (A) & from an inactive RR-MS ...read more
Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 2-DZ analysis & identification of MMP-9 by Western blot. Left panels (A & B): 2-D zymography (2-DZ) of serum samples from healthy control (A) & from an inactive RR-MS ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Eq, Pm, RbSpecies Glossary
Applications WB, ELISA, IHC
Clone
4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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MMP-9 Antibody (4A3) Summary

Immunogen
Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Localization
Secreted > extracellular space > extracellular matrix
Specificity
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
Predicted Species
Porcine (100%), Rabbit (100%), Primate (100%), Equine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:500-1:1000
Application Notes
In Western blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 3 Reviews rated 3.7
using
NBP2-13173 in the following applications:

Publications
Read Publications using
NBP2-13173 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32725911). Mouse reactivity reported in scientific literature (PMID: 30772382). .

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MMP-9 Antibody (4A3)

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-9 Antibody (NBP2-13173)(36)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 8 applications: CyTOF-ready, IF/IHC, IHC, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, WB, Western Blot.


Filter By Application
CyTOF-ready
(1)
IF/IHC
(1)
IHC
(1)
IHC-P
(6)
Immunocytochemistry/ Immunofluorescence
(1)
Immunohistochemistry-Paraffin
(1)
WB
(14)
Western Blot
(1)
All Applications
Filter By Species
Human
(9)
Mouse
(4)
Rat
(10)
Canine
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using NBP2-13173 Applications Species
Francesca Ferrara, Xi Yan, Alessandra Pecorelli, Anna Guiotto, Sante Colella, Arianna Pasqui, John Ivarrson, Stephen Lynch, Sara Anderias, Hina Choundhary, Stacy White, Giuseppe Valacchi Combined exposure to UV and PM affect skin oxinflammatory responses and it is prevented by antioxidant mix topical application: Evidences from clinical study. Journal of cosmetic dermatology 2024-04-08 [PMID: 38590207]
Elisabetta Esposito, Francesca Ferrara, Markus Drechsler, Olga Bortolini, Daniele Ragno, Sofia Toldo, Agnese Bondi, Alessandra Pecorelli, Rebecca Voltan, Paola Secchiero, Giorgio Zauli, Giuseppe Valacchi, Ramón Cacabelos Nutlin-3 Loaded Ethosomes and Transethosomes to Prevent UV-Associated Skin Damage Life 2024-01-21 [PMID: 38276284]
Liu D, Wang T, Wang Q et al. Identification of key genes in sepsis-induced cardiomyopathy based on integrated bioinformatical analysis and experiments in vitro and in vivo PeerJ 2023-11-21 [PMID: 38025678] (WB, Mouse) WB Mouse
Sun DP, Huang HY, Chou CL et al. Punicalagin is cytotoxic to human colon cancer cells by modulating cell proliferation, apoptosis, and invasion Human & experimental toxicology 2023-11-07 [PMID: 37933160]
Pambianchi E, Hagenberg Z, Pecorelli A et al. Tension as a key factor in skin responses to pollution Sci Rep 2023-09-25 [PMID: 37749125]
Abbaszadeh F, Jorjani M, Joghataei MT et al. Astaxanthin ameliorates spinal cord edema and astrocyte activation via suppression of HMGB1/TLR4/NF-?B signaling pathway in a rat model of spinal cord injury Naunyn-Schmiedeberg's archives of pharmacology 2023-05-05 [PMID: 37145127] (WB, Rat) WB Rat
Cheng Y, Li J, Zhang X et al. Protective Effect of Qingchang Wenzhong Decoction on Colitis and Colitis-Related Carcinogenesis by Regulating Inflammation and Intestinal Fibrosis Journal of inflammation research 2023-04-07 [PMID: 37056910] (IHC-P, Mouse) IHC-P Mouse
Guo X, Sun Y, Wang Z et al. The Preventive Effect of A Magnetic Nanoparticle-Modified Root Canal Sealer on Persistent Apical Periodontitis International journal of molecular sciences 2022-10-28 [PMID: 36361925] (IHC-P, Canine)

Details:
Dilution used in IHC-P 1:300
IHC-P Canine
Bhadwal P, Randhawa V, Vaiphei K et al. Clinical relevance of CERK and SPHK1 in breast cancer and their association with metastasis and drug resistance Scientific reports 2022-10-29 [PMID: 36309544] (WB, Human)

Details:
Dilution used in WB 1:1000
WB Human
Radnaa E, Richardson L, Goldman B Et al. Stress signaler p38 mitogen-activated kinase activation: a cause for concern? Clin Sci (Lond) 2022-10-17 [PMID: 36250628] (CyTOF-ready, Human)

Details:
Citation using the Azide and BSA Free version of this antibody.
CyTOF-ready Human
Show All 36 Publications.

Reviews for MMP-9 Antibody (NBP2-13173) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rat.

Reviews using NBP2-13173:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Shinford Sun
WB Human 05/24/2024
View

Summary

ApplicationWestern Blot
Sample TestedHuman lung tumor
SpeciesHuman
LotD108660-1
Western Blot MMP-9 NBP2-13173
Enlarge
4
reviewed by:
Verified Customer
WB Rat 07/20/2016
View

Summary

ApplicationWestern Blot
Sample Testedrat smooth muscle
SpeciesRat
LotA-2
Western Blot MMP-9 NBP2-13173
Enlarge
3
reviewed by:
Kristina Knubel
WB Human 02/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedU251 glioma cell line; whole cell lysate
SpeciesHuman

Product General Protocols

View specific protocols for MMP-9 Antibody (NBP2-13173): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (NBP2-13173). (Showing 1 - 2 of 2 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.
  2. Would it be possible for you to send me your validation data on MMP9 Antibody IIA5 NBP1-40248? I am considering using it in my research project on colorectal cancer liver metastases.
    • Our MMP9 Antibody IIA5 NBP1-40248 was validated in Western blot through external validation, and unfortunately, I do not have an images to share at the moment. NBP2-13173 is another very similar monoclonal antibody validated in human and rat for use in Western blot. We do have images from in-house testing for this product (please see the datasheet for NBP2-13173, under the images tab), so you may be more comfortable ordering this antibody for this target.

Secondary Antibodies

 

Isotype Controls

Additional MMP-9 Products

Research Areas for MMP-9 Antibody (NBP2-13173)

Find related products by research area.

Blogs on MMP-9.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Recent Reviews

3.7
5
0
4
2
3
1
2
0
1
0

Shinford Sun
05/24/2024
Application: WB
Species: Human

Verified Customer
07/20/2016
Application: WB
Species: Rat

Kristina Knubel
02/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MMP9
Entrez
Uniprot