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MMP-3 Antibody (10D6) [Unconjugated]

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MMP-3 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB905) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP-3 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB905) overnight at 4 °C. Tissue was stained with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
10D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB905-SP
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MMP-3 Antibody (10D6) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-3
Specificity
Detects the pro and active forms of human MMP-3 in Western blots. In Western blots, no cross-reactivity with recombinant human
(rh) MMP‑1, rhMMP-2 or rhMMP-9 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB905 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-3 Antibody (10D6) [Unconjugated]

  • CHDS6
  • EC 3.4.24
  • EC 3.4.24.17
  • matrix metallopeptidase 3 (stromelysin 1, progelatinase)
  • matrix metalloproteinase 3 (stromelysin 1, progelatinase)
  • Matrix metalloproteinase-3
  • MGC126102
  • MGC126103
  • MMP3
  • MMP-3
  • proteoglycanase
  • SL-1
  • STMY
  • STMY1MGC126104
  • STR1
  • Stromelysin 1
  • stromelysin-1
  • transin-1

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha  chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta , IGFBP-3, pro MMP-1, pro MMP-7, pro MMP-8, pro MMP-9 and pro MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MMP-3 Antibody
(10D6) [Unconjugated]
MAB905
MMP-3 Antibody (10D6) [Unconjugated]
Species: Hu
Applications: WB, IHC, IP

Publications for MMP-3 Antibody (MAB905)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Dot Blot, Western Blot.


Filter By Application
Dot Blot
(1)
Western Blot
(4)
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Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB905 Applications Species
J Kudelski, G M?ynarczyk, M Gudowska-S, B Mroczko, B Darewicz, M Bruczko-Go, L Romanowicz Higher Content but Not Activity of Stromelysin-2 (MMP-10) in Comparison to Stromelysin-1 (MMP-3) in Human Renal Carcinoma International Journal of Environmental Research and Public Health, 2022-10-02;19(19):. 2022-10-02 [PMID: 36231910] (Western Blot, Human) Western Blot Human
J Kudelski, G M?ynarczyk, M Gudowska-S, B Mroczko, B Darewicz, M Bruczko-Go, K Sobolewski, L Romanowicz Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma Journal of Clinical Medicine, 2021-08-19;10(16):. 2021-08-19 [PMID: 34441979] (Western Blot, Human) Western Blot Human
Kong MY, Gaggar A, Li Y Matrix metalloproteinase activity in pediatric acute lung injury. Int J Med Sci, 2008-12-16;6(1):9-17. 2008-12-16 [PMID: 19159011] (Western Blot, Human) Western Blot Human
Naveau A, Reinald N, Fournier B Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model. Connect. Tissue Res., 2007-01-01;48(6):300-8. 2007-01-01 [PMID: 18075816] (Dot Blot, Human) Dot Blot Human
Liu Z, Ivanoff A, Klominek J Expression and activity of matrix metalloproteases in human malignant mesothelioma cell lines. Int J Cancer, 2001-03-01;91(5):638-43. 2001-03-01 [PMID: 11267973] (Western Blot, Human) Western Blot Human

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MMP3