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MMP-13 Antibody (OTI2D8) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
OTI2D8
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72740AF350
Alexa Fluor 405
NBP2-72740AF405
Alexa Fluor 488
NBP2-72740AF488
Alexa Fluor 532
NBP2-72740AF532
Alexa Fluor 594
NBP2-72740AF594
Alexa Fluor 647
NBP2-72740AF647
Alexa Fluor 700
NBP2-72740AF700
Alexa Fluor 750
NBP2-72740AF750
Allophycocyanin
NBP2-72740APC
Biotin
NBP2-72740B
DyLight 350
NBP2-72740UV
DyLight 405
NBP2-72740V
DyLight 488
NBP2-72740G
DyLight 550
NBP2-72740R
DyLight 594
NBP2-72740DL594
DyLight 650
NBP2-72740C
DyLight 680
NBP2-72740FR
DyLight 755
NBP2-72740IR
FITC
NBP2-72740F
HRP
NBP2-72740H
Janelia Fluor 525
NBP2-72740JF525
Janelia Fluor 549
NBP2-72740JF549
Janelia Fluor 585
NBP2-72740JF585
Janelia Fluor 635
NBP2-72740JF635
Janelia Fluor 646
NBP2-72740JF646
Janelia Fluor 669
NBP2-72740JF669
PE
NBP2-72740PE
PerCP
NBP2-72740PCP
Unconjugated
NBP2-45887
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45887
Azide and BSA Free
NBP2-72740
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MMP-13 Antibody (OTI2D8) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Human recombinant protein fragment corresponding to amino acids 104-471 of human MMP13 (NP_002418) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MMP13
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MMP-13 Antibody (OTI2D8) [CoraFluor™ 1]

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP13