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MMP-13 Antibody (OTI2D8) - Azide and BSA Free

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Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.
Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.
Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Anti-MMP13 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ...read more
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of extracts (10ug) from 5 different cell lines by using anti-MMP13 monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. ...read more
Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. ...read more
Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. ...read more
Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced ...read more
Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced ...read more
Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Human lymph node tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by ...read more
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
OTI2D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72740AF350
Alexa Fluor 405
NBP2-72740AF405
Alexa Fluor 488
NBP2-72740AF488
Alexa Fluor 532
NBP2-72740AF532
Alexa Fluor 594
NBP2-72740AF594
Alexa Fluor 647
NBP2-72740AF647
Alexa Fluor 700
NBP2-72740AF700
Alexa Fluor 750
NBP2-72740AF750
Allophycocyanin
NBP2-72740APC
Biotin
NBP2-72740B
DyLight 350
NBP2-72740UV
DyLight 405
NBP2-72740V
DyLight 488
NBP2-72740G
DyLight 550
NBP2-72740R
DyLight 594
NBP2-72740DL594
DyLight 650
NBP2-72740C
DyLight 680
NBP2-72740FR
DyLight 755
NBP2-72740IR
FITC
NBP2-72740F
HRP
NBP2-72740H
Janelia Fluor 549
NBP2-72740JF549
Janelia Fluor 646
NBP2-72740JF646
PE
NBP2-72740PE
PerCP
NBP2-72740PCP
Unconjugated
NBP2-45887
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45887
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MMP-13 Antibody (OTI2D8) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 104-471 of human MMP13 (NP_002418) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MMP13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:4000
Theoretical MW
42.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for MMP-13 Antibody (OTI2D8) - Azide and BSA Free

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MMP-13 Antibody
(OTI2D8) - Azide and ...
NBP2-72740
MMP-13 Antibody (OTI2D8) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, KD

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP13