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MMP-13 Antibody (87518) [Unconjugated] - Pro form

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Recombinant Human Pro-MMP‑13 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB5111) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro MMP‑13 Monoclonal Antibody (Catalog # MAB913) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant Human Pro-MMP‑13 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB5111) coated on a Clear Polystyrene Microplate (Catalog # DY990). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP, AP
Clone
87518
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB913-SP
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MMP-13 Antibody (87518) [Unconjugated] - Pro form Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452
Specificity
Detects human Pro MMP‑13 in direct ELISAs and Western blots. Does not detect active recombinant human MMP-13. In Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MMP13
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB913 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-13 Antibody (87518) [Unconjugated] - Pro form

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-13 Antibody (MAB913)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: Immunoprecipitation.


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Bioinformatics

Gene Symbol MMP13
Uniprot