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MMP-12 Antibody [Unconjugated]

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Western blot shows mouse serum and mouse plasma. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse MMP-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3467) followed by HRP-conjugated Anti-Goat IgG ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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MMP-12 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP-12
Ala18-Cys462
Accession # EDL24933
Specificity
Detects mouse MMP-12 in direct ELISAs and Western blots. In direct ELISAs,less than 20% cross-reactivity with recombinant human MMP‑12 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-8, and rmMMP-10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP12
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
AF3467 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-12 Antibody [Unconjugated]

  • EC 3.4.24
  • hME
  • HMEEC 3.4.24.65
  • Macrophage elastase
  • macrophage metalloelastase
  • matrix metallopeptidase 12 (macrophage elastase)
  • matrix metalloproteinase 12 (macrophage elastase)
  • Matrix metalloproteinase-12
  • ME
  • MGC138506
  • MME
  • MMP12
  • MMP-12

Background

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-12 (macrophage elastase) is found in macrophages and its expression in monocytes can be induced by cytokines such as GM-CSF and CD40 signaling. In addition to elastin, MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. MMP-12 is required for macrophage-mediated proteolysis and matrix invasion in mice. MMP-12 is proposed to have a direct role in the pathogenesis of aortic aneurysms and in the development of pulmonary emphysema that results from chronic inhalation of cigarette smoke. Structurally, the pro-MMP-12 consists of following domains: a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. The recombinant mouse MMP-12 corresponds to the pro form that can be activated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-12 Antibody (AF3467)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Western Blot
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Product General Protocols

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FAQs for MMP-12 Antibody (AF3467) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MMP12
Uniprot