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MHC Class I Antibody (MEM-E/06) - BSA Free

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Immunohistochemistry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).
Flow Cytometry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Analysis using PE conjugate of NB500-307. A cell surface stain was performed on THP-1 cells with MHC Class I antibody (MEM-E/06) NB500-307 (blue) and a ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, IHC, IP, CyTOF-ready
Clone
MEM-E/06
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

MHC Class I Antibody (MEM-E/06) - BSA Free Summary

Immunogen
Bacterially expressed recombinant full length protein refolded with beta2-microglobulin and peptide (Human).
Localization
Type I membrane protein.
Specificity
This antibody (clone MEM-E/06) recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. Some resultss showed that this antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, others have confirmed that this antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-E
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
Application Notes
This antibody is Cytof ready.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for MHC Class I Antibody (MEM-E/06) - BSA Free

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1

Background

HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HLA-E
Entrez