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DAP Kinase 1 Antibody (SR1225)

Images

 
Immunohistochemistry: DAP Kinase 1 Antibody (SR1225) [NBP3-21649] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21649.
Western Blot: DAP Kinase 1 Antibody (SR1225) [NBP3-21649] - Western blot analysis using NBP3-21649 in HeLa cell lysate.
Immunocytochemistry/ Immunofluorescence: DAP Kinase 1 Antibody (SR1225) [NBP3-21649] - Immunofluorescent analysis of Hela cells, using NBP3-21649.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SR1225
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DAP Kinase 1 Antibody (SR1225) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human DAP Kinase 1 (Uniprot #: P53355)
Specificity
Detects endogenous levels of total DAP Kinase 1
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
DAPK1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for DAP Kinase 1 Antibody (SR1225)

  • DAP Kinase 1
  • DAPK
  • DAPK1
  • DAPKDAP kinase 1
  • death-associated protein kinase 1
  • DKFZp781I035
  • EC 2.7.11
  • EC 2.7.11.1

Background

DAPK1 (Death-associated protein kinase 1) is a calmodulin dependent serine/threonine serine kinase which functions as a positive mediator of gamma-interferon induced programmed cell death. DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAP Kinase 1 Antibody (NBP3-21649) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DAP Kinase 1 Antibody (NBP3-21649) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DAPK1