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MGMT Antibody (MT 23.2) [CoraFluor™ 1]

 
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Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, CyTOF-ready
Clone
MT 23.2
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-168AF350
Alexa Fluor 405
NB100-168AF405
Alexa Fluor 488
NB100-168AF488
Alexa Fluor 532
NB100-168AF532
Alexa Fluor 594
NB100-168AF594
Alexa Fluor 647
NB100-168AF647
Alexa Fluor 700
NB100-168AF700
Alexa Fluor 750
NB100-168AF750
Allophycocyanin
NB100-168APC
Biotin
NB100-168B
DyLight 350
NB100-168UV
DyLight 405
NB100-168V
DyLight 488
NB100-168G
DyLight 550
NB100-168R
DyLight 594
NB100-168DL594
DyLight 650
NB100-168C
DyLight 680
NB100-168FR
DyLight 755
NB100-168IR
FITC
NB100-168F
HRP
NB100-168H
Janelia Fluor 525
NB100-168JF525
Janelia Fluor 549
NB100-168JF549
Janelia Fluor 646
NB100-168JF646
Janelia Fluor 669
NB100-168JF669
PE
NB100-168PE
PerCP
NB100-168PCP
Unconjugated
NB100-168
mFluor Violet 450 SE
NB100-168MFV450
mFluor Violet 500 SE
NB100-168MFV500
mFluor Violet 610 SE
NB100-168MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-168
BSA Free
NB100-168SS
Azide and BSA Free
NBP2-80847
BSA Free
NB100-168
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MGMT Antibody (MT 23.2) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455]
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MGMT
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

This antibody is mouse reactivity negative.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MGMT Antibody (MT 23.2) [CoraFluor™ 1]

  • 6-O-methylguanine-DNA methyltransferase
  • EC 2.1.1.63
  • methylated-DNA--protein-cysteine methyltransferase
  • methylguanine-DNA methyltransferase
  • MGMT
  • O-6-methylguanine-DNA methyltransferase
  • O6-methylguanine-DNA methyltransferase
  • O-6-methylguanine-DNA-alkyltransferase

Background

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MGMT