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MEK1 Antibody

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Western Blot: MEK1 Antibody [NBP2-17265] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE gel, diluted at 1:2000.
Western Blot: MEK1 Antibody [NBP2-17265] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP2-17265] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MEK1+2 protein stained by MEK1+2 antibody diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: MEK1 Antibody [NBP2-17265] - Paraffin-embedded rat liver. MEK1+2 antibody diluted at 1:500.
Western Blot: MEK1 Antibody [NBP2-17265] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Bv, Ca, Ch, Pm, Rb, RM, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MEK1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MEK1. The exact sequence is proprietary.
Predicted Species
Bovine (100%), Rabbit (100%), Zebrafish (94%), Rhesus Macaque (100%), Chicken (98%), Canine (100%), Xenopus (96%), Primate (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP2K1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-17265 in the following applications:

  • WB
    1 publication

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31757483).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for MEK1 Antibody

  • dual specificity mitogen-activated protein kinase kinase 1
  • ERK activator kinase 1
  • MAP kinase kinase 1
  • MAP2K1
  • MAPK/ERK kinase 1
  • MAPKK1
  • MEK1
  • MEK1MAPKK 1
  • mitogen-activated protein kinase kinase 1
  • MKK1
  • MKK1MEK 1
  • PRKMK1EC 2.7.12.2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-17265
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MEK1 Antibody (NBP2-17265)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAP2K1
Uniprot