Western Blot: MEK1 Antibody [NBP2-17265] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP2-17265] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MEK1+2 protein stained by MEK1+2 antibody diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: MEK1 Antibody [NBP2-17265] - Paraffin-embedded rat liver. MEK1+2 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP2-17265] - MEK1 antibody detects MEK1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT ...read more
Western Blot: MEK1 Antibody [NBP2-17265] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (NBP2-17265) diluted at 1:1000. The HRP-conjugated ...read more
Western Blot: MEK1 Antibody [NBP2-17265] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (NBP2-17265) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG ...read more
Product Details
Summary
Reactivity
Hu, Mu, Rt, Ca, Bv, Ca, Ch, Pm, Rb, RM, Xp, ZeSpecies Glossary
43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-17265 in the following applications:
Porcine reactivity reported in scientific literature (PMID: 31757483).
Packaging, Storage & Formulations
Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified
Alternate Names for MEK1 Antibody
dual specificity mitogen-activated protein kinase kinase 1
ERK activator kinase 1
MAP kinase kinase 1
MAP2K1
MAPK/ERK kinase 1
MAPKK1
MEK1
MEK1MAPKK 1
mitogen-activated protein kinase kinase 1
MKK1
MKK1MEK 1
PRKMK1EC 2.7.12.2
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Background
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Species: Hu, Mu, Rt, Ca, Bv, Ca, Ch, Pm, Rb, RM, Xp, Ze
Applications: WB, ICC/IF, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for MEK1 Antibody (NBP2-17265)(1)
We have publications tested in 1 confirmed species: Porcine.
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