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Mcl-1 Antibody (OTI10F6) - Azide and BSA Free

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Western Blot: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.
Western Blot: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MCL-1.
Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse ...read more
Western Blot: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI10F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72598AF350
Alexa Fluor 405
NBP2-72598AF405
Alexa Fluor 488
NBP2-72598AF488
Alexa Fluor 532
NBP2-72598AF532
Alexa Fluor 594
NBP2-72598AF594
Alexa Fluor 647
NBP2-72598AF647
Alexa Fluor 700
NBP2-72598AF700
Alexa Fluor 750
NBP2-72598AF750
Allophycocyanin
NBP2-72598APC
Allophycocyanin/Cy7
NBP2-72598APCCY7
Biotin
NBP2-72598B
CoraFluor 1
NBP2-72598CL1
DyLight 350
NBP2-72598UV
DyLight 405
NBP2-72598V
DyLight 488
NBP2-72598G
DyLight 550
NBP2-72598R
DyLight 594
NBP2-72598DL594
DyLight 650
NBP2-72598C
DyLight 680
NBP2-72598FR
DyLight 755
NBP2-72598IR
FITC
NBP2-72598F
HRP
NBP2-72598H
Janelia Fluor 525
NBP2-72598JF525
Janelia Fluor 549
NBP2-72598JF549
Janelia Fluor 585
NBP2-72598JF585
Janelia Fluor 635
NBP2-72598JF635
Janelia Fluor 646
NBP2-72598JF646
Janelia Fluor 669
NBP2-72598JF669
PE
NBP2-72598PE
PE/Atto594
NBP2-72598PEATT594
PE/Cy5.5
NBP2-72598PECY55
PE/Cy7
NBP2-72598PECY7
PerCP
NBP2-72598PCP
Unconjugated
NBP2-45773
mFluor Violet 450 SE
NBP2-72598MFV450
mFluor Violet 500 SE
NBP2-72598MFV500
mFluor Violet 610 SE
NBP2-72598MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45773
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Mcl-1 Antibody (OTI10F6) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MCL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200 - 1:1000
Theoretical MW
37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM

Background

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Mcl-1 Antibody
(OTI10F6) - Azide and ...
NBP2-72598
Mcl-1 Antibody (OTI10F6) - Azide and BSA Free
Species: Hu
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MCL1