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Mcl-1 Antibody (CL1128)

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Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MCL1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL1128
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

  • Size / Catalog#QtyPrice
    NBP2-52968-25ul
    25ul
    $359.00
    4-6 Business Days
    NBP2-52968
    0.1 ml
    $549.00
    4-6 Business Days
Shipping $45.00 per order
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Reviews & Publications
Protocols & FAQs
Support & Research

Mcl-1 Antibody (CL1128) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDG
Epitope
PEEELDGYEP
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MCL1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Control Peptide
Mcl-1 Recombinant Protein Antigen (NBP2-52968PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-52968 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Mcl-1 Antibody (CL1128)

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM

Background

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recent Reviews

5
5
1
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0
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0
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1
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Kristen Gumz
04/28/2017
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol MCL1