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MBNL3 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: MBNL3 Antibody [NBP1-85635] - Staining in human placenta and prostate tissues using anti-MBNL3 antibody. Corresponding MBNL3 RNA-seq data are presented for ...read more
Western Blot: MBNL3 Antibody [NBP1-85635] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Hep-G2
Immunocytochemistry/ Immunofluorescence: MBNL3 Antibody [NBP1-85635] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MBNL3 Antibody [NBP1-85635] - Staining of human placenta shows high expression.
Immunohistochemistry-Paraffin: MBNL3 Antibody [NBP1-85635] - Staining of human prostate shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

MBNL3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AQMSSLGSFPMTPSIPANPPMAFNPYIPHPGMGLVPAELVPNTPVLIPGNPPLAMPGAVGPKLMRSDKLEVCREFQRGNCTRGENDCRYAHPTDASMIEASDNTVTICMDYIKGRCSREKCKYFHPPAHLQARLKAAHHQMNHSAAS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MBNL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MBNL3 Protein (NBP1-85635PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MBNL3 Antibody

  • CHCRMBLX39FLJ11316
  • Cys3His CCG1-required protein
  • FLJ97142
  • MBXLMBLX
  • muscleblind-like 3 (Drosophila)
  • muscleblind-like protein 3
  • Muscleblind-like X-linked protein
  • Protein HCHCR

Background

The MBNL3 gene encodes a member of the muscleblind-like family of proteins. The encoded protein may function in regulation of alternative splicing and may play a role in the pathophysiology of myotonic dystrophy. Alternatively spliced transcript variants have

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MBNL3