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Recombinant Human MAT1A His Protein

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SDS-Page: Recombinant Human MAT1A Protein [NBP1-40408]
SDS-Page: Recombinant Human MAT1A Protein [NBP1-40408]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human MAT1A His Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-395 of Human MAT1A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MNGPVDGLCD HSLSEGVFMF TSESVGEGHP DKICDQISDA VLDAHLKQDP NAKVACETVC KTGMVLLCGE ITSMAMVDYQ RVVRDTIKHI GYDDSAKGFD FKTCNVLVAL EQQSPDIAQC VHLDRNEEDV GAGDQGLMFG YATDETEECM PLTIILAHKL NARMADLRRS GLLPWLRPDS KTQVTVQYMQ DNGAVIPVRI HTIVISVQHN EDITLEEMRR ALKEQVIRAV VPAKYLDEDT VYHLQPSGRF VIGGPQGDAG VTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW VAKSLVKAGL CRRVLVQVSY AIGVAEPLSI SIFTYGTSQK TERELLDVVH KNFDLRPGVI VRDLDLKKPI YQKTACYGHF GRSEFPWEVP RKLVF
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
MAT1A
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
45.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 0.1 M NaCl, 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human MAT1A His Protein

  • AdoMet synthase 1
  • adoMet synthetase 1
  • AMS1
  • MAT
  • MATA1MAT 1
  • MAT-I/III
  • Methionine adenosyltransferase 1
  • methionine adenosyltransferase I, alpha
  • Methionine adenosyltransferase I/III
  • S-adenosylmethionine synthase isoform type-1
  • S-adenosylmethionine synthetase isoform type-1
  • SAMS
  • SAMS1EC 2.5.1.6

Background

MAT1A catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver. MAT1A expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT2A present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NFkappaB and TNFalpha cause a switch from MAT1A to MAT2A expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth. Recombinant human MAT1A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol MAT1A