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MAGP-2/MFAP5 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining in human smooth muscle and cerebral cortex tissues using anti-MFAP5 antibody. Corresponding MFAP5 RNA-seq data ...read more
Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803] - Analysis in human cell line BEWO.
Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human smooth muscle shows high expression.
Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human rectum shows strong membranous positivity in cells in lamina propria.
Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human testis shows strong membranous positivity in cells in lamina propria.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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MAGP-2/MFAP5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GVNSQRGDDVTQATPETFTEDPNLVNDPATDETVLAVLADIAPSTDDLASLSEKNTTAECWDEKFTCTRLYSVHRPVKQCIHQLCFTSLRRMYIVNKEICSRLVCKEHEAMKDELCRQMAGLPPRRLRRSNYFRLPPCENV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MFAP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
MAGP-2/MFAP5 Protein (NBP1-82803PEP)
Publications
Read Publication using
NBP1-82803 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (83%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAGP-2/MFAP5 Antibody

  • MAGP2
  • MAGP-2
  • MAGP2MFAP-5
  • MFAP5
  • Microfibril-associated glycoprotein 2
  • microfibrillar associated protein 5
  • microfibrillar-associated protein 5
  • MP25
  • MP25microfibril-associated glycoprotein-2

Background

MFAP5, also known as Microfibrillar-associated protein 5, is a 173 amino acid protein that is 20 kDa, extracellular matrix secreted, and it is a component of the elastin-associated microfibrils. Current research is being performed on this protein involvement in marfan syndrome, scleroderma and skin fibrosis. The protein is involved in molecules associated with elastic fibres, extracellular matrix organization, elastic fibre formation, tropoelastin associates with microfibrils, elastin cross-linking by lysyl oxidase, and notch signaling pathways where it interacts with FBN2, JAG1, JAG2, MEGF6, VWF, FBLN1, FBN1, GYG1, DLL1, MFAP2, NOTCH1, ENAM, FBN3, and DLL4 proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAGP-2/MFAP5 Antibody (NBP1-82803)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


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IF/IHC
(1)
WB
(1)
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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol MFAP5