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Tsukushi/TSK Antibody

Images

 
Genetic Strategies: Western Blot: Tsukushi/TSK Antibody [NBP1-87959] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human cell line A549 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Western Blot: Tsukushi/TSK Antibody [NBP1-87959] - Analysis in human cell line A-549.
Western Blot: Tsukushi/TSK Antibody [NBP1-87959] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Tsukushi/TSK Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSKU
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Tsukushi/TSK Protein (NBP1-87959PEP)
Publications
Read Publication using NBP1-87959.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Tsukushi/TSK Antibody

  • E2IG4
  • E2IG4E2-induced gene 4 protein
  • leucine rich repeat containing 54
  • Leucine-rich repeat-containing protein 54
  • LRRC54
  • TSK
  • TSKtsukushi homolog
  • TSKU
  • tsukushi small leucine rich proteoglycan homolog (Xenopus laevis)
  • Tsukushi
  • tsukushi, small leucine rich proteoglycan
  • tsukushin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tsukushi/TSK Antibody (NBP1-87959)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tsukushi/TSK Antibody (NBP1-87959) (0)

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Bioinformatics

Gene Symbol TSKU