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M-CSFR/CD115 Antibody [Unconjugated]

 (11 Publications)
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M-CSF R/CD115 was detected in immersion fixed mouse splenocytes using Mouse M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3818) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
M-CSF R/CD115 was detected in immersion fixed mouse splenocytes using Mouse M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3818) at 15 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3818
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3818-SP
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M-CSFR/CD115 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse M-CSF R/CD115
Ala20-Ser511
Accession # P09581
Specificity
Detects mouse M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human M-CSF R/CD115 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CSF1R
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3818 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSFR/CD115 Antibody [Unconjugated]

  • CD115 antigen
  • CD115
  • c-fms
  • colony stimulating factor 1 receptor
  • CSF1R
  • CSF-1-R
  • CSFR
  • EC 2.7.10.1
  • FMS proto-oncogene
  • FMSFIM2
  • macrophage colony stimulating factor I receptor
  • macrophage colony-stimulating factor 1 receptor
  • McDonough feline sarcoma viral (v-fms) oncogene homolog
  • M-CSF R
  • MCSFR
  • M-CSFR
  • Proto-oncogene c-Fms

Background

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares > 99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

  1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
  2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
  3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
  5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
  6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
  7. Dai, X. et al. (2002) Blood 99:111.
  8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  9. Li, M. et al. (2004) J. Neurochem. 91:623.
  10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for M-CSFR/CD115 Antibody (AF3818)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Immunoprecipitation.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(3)
IHC-Fr
(1)
Immunoprecipitation
(1)
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Filter By Species
Mouse
(7)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF3818 Applications Species
Li, X;Hu, B;Guan, X;Wang, Z;Zhou, Y;Sun, H;Zhang, X;Li, Y;Huang, X;Zhao, Y;Wang, X;Xu, H;Zhang, YW;Wang, Z;Zheng, H; Minocycline protects against microgliopathy in a Csf1r haplo-insufficient mouse model of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) Journal of neuroinflammation 2023-05-31 [PMID: 37259140] (Mouse) Mouse
Rafael S. Czepielewski, Emma C. Erlich, Emily J. Onufer, Shannon Young, Brian T. Saunders, Yong-Hyun Han et al. Ileitis-associated tertiary lymphoid organs originate at lymphatic valves and obstruct mesenteric lymph flow in response to tumor necrosis factor Immunity 2021-12-01 [PMID: 34788601]
M Zelic, F Pontarelli, L Woodworth, C Zhu, A Mahan, Y Ren, M LaMorte, R Gruber, A Keane, P Loring, L Guo, TH Xia, B Zhang, P Orning, E Lien, A Degterev, T Hammond, D Ofengeim RIPK1 activation mediates neuroinflammation and disease progression in multiple sclerosis Cell Reports, 2021-05-11;35(6):109112. 2021-05-11 [PMID: 33979622] (ICC, Mouse) ICC Mouse
D Nishida, A Arai, L Zhao, M Yang, Y Nakamichi, K Horibe, A Hosoya, Y Kobayashi, N Udagawa, T Mizoguchi RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp Scientific Reports, 2021-02-25;11(1):4575. 2021-02-25 [PMID: 33633362] (Flow Cytometry, IHC, Mouse) Flow Cytometry, IHC Mouse
Pons V, Levesque P, Plante M, Rivest S Conditional genetic deletion of CSF1 receptor in microglia ameliorates the physiopathology of Alzheimer's disease. Alzheimers Res Ther, 2021-01-05;13(1):8. 2021-01-05 [PMID: 33402196] (IHC, Mouse) IHC Mouse
Ayman M. Ibrahim, Matthew A. Moss, Zane Gray, Michelle D. Rojo, Caitlin M. Burke, Kathryn L. Schwertfeger et al. Diverse Macrophage Populations Contribute to the Inflammatory Microenvironment in Premalignant Lesions During Localized Invasion Frontiers in Oncology 2020-09-24 [PMID: 33072601]
PL Wang, AKY Yim, KW Kim, D Avey, RS Czepielews, M Colonna, J Milbrandt, GJ Randolph Peripheral nerve resident macrophages share tissue-specific programming and features of activated microglia Nat Commun, 2020-05-21;11(1):2552. 2020-05-21 [PMID: 32439942] (IHC-Fr, Mouse) IHC-Fr Mouse
S Davidson, M Efremova, A Riedel, B Mahata, J Pramanik, J Huuhtanen, G Kar, R Vento-Torm, T Hagai, X Chen, MA Haniffa, JD Shields, SA Teichmann Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth Cell Rep, 2020-05-19;31(7):107628. 5/19/2020 [PMID: 32433953]
CM Marsters, D Nesan, R Far, N Klenin, QJ Pittman, DM Kurrasch Embryonic microglia influence developing hypothalamic glial populations J Neuroinflammation, 2020-05-06;17(1):146. 2020-05-06 [PMID: 32375817] (IHC, Mouse) IHC Mouse
Vincent Pons, Nataly Laflamme, Paul Préfontaine, Serge Rivest Role of Macrophage Colony-Stimulating Factor Receptor on the Proliferation and Survival of Microglia Following Systemic Nerve and Cuprizone-Induced Injuries Frontiers in Immunology 2020-01-29 [PMID: 32082318]
Show All 11 Publications.

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Bioinformatics

Gene Symbol CSF1R
Uniprot