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3 Results for "m-csfr-cd115" in ELISA Kits
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DY329

Human M-CSF R/CD115 DuoSet ELISA, 15 Plate

(3 Publications)
1 Kit (for 15 Plates)
DY329
N/A M-CSFR/CD115 [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
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DYC3268-2

Human Phospho-M-CSF R DuoSet IC ELISA, 2 Plate

(1 Publication)
1 Kit (for 2 Plates)
DYC3268-2
The human acute monocytic leukemia cell line THP-1 was treated with 600 ng/mL recombinant human M-CSF (R&D Systems, Catalog #216-MC) for five minutes to induce tyrosine phosphorylation of M-CSF Receptor. Lysates were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-M-CSF Receptor polyclonal antibody and protein G agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (R&D Systems, Catalog # BAM1676) to detect phospho-M-CSF Receptor. Bands were visualized with Streptavidin-HRP (R&D Systems, Cat # DY998) followed by chemiluminescent detection using WesternGloTM Chemiluminescent Detection Substrate (R&D Systems, Catalog # AR004). Human Phospho-M-CSF Receptor can be detected in this DuoSet IC ELISA by using approximately 4 to 8 times less lysate than is needed for a conventional IP-Western blot.
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC3268E
Biotin DYC3268-5
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KA2218

Human M-CSFR/CD115 ELISA Kit (Colorimetric)

1 Kit
Hu
Species Glossary
Reactivity: Hu
ELISA, NULL
Applications: ELISA, NULL
63 to 4000 pg/mL
n/a
Cell Lysate, Cell Supernatants, Plasma, Serum
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