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M-CSFR/CD115 Antibody (61701) [Unconjugated]

 (11 Publications)
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Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) followed by HRP-conjugated ...read more
Recombinant Human M-CSF R/CD115 Fc Chimera (329-MR) inhibits Recombinant Human M-CSF (Catalog # 216-MC) induced proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner ...read more
M-CSF R/CD115 was detected in immersion fixed paraffin-embedded sections of Human Spleen using Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) at 15 µg/mL for 1 hour at room temperature ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clone
61701
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3291-SP
Carrier Free
MAB3291-500
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M-CSFR/CD115 Antibody (61701) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115
Ile20-Glu512 (Pro54Ala)
Accession # P07333.2
Specificity
Detects human M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSF1R
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 4-16 ng/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB3291 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSFR/CD115 Antibody (61701) [Unconjugated]

  • CD115 antigen
  • CD115
  • c-fms
  • colony stimulating factor 1 receptor
  • CSF1R
  • CSF-1-R
  • CSFR
  • EC 2.7.10.1
  • FMS proto-oncogene
  • FMSFIM2
  • macrophage colony stimulating factor I receptor
  • macrophage colony-stimulating factor 1 receptor
  • McDonough feline sarcoma viral (v-fms) oncogene homolog
  • M-CSF R
  • MCSFR
  • M-CSFR
  • Proto-oncogene c-Fms

Background

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin  alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

  1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
  2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
  3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
  5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
  6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
  7. Dai, X. et al. (2002) Blood 99:111.
  8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  9. Li, M. et al. (2004) J. Neurochem. 91:623.
  10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for M-CSFR/CD115 Antibody (MAB3291)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: Bioassay, Functional Assay, ICC, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
Functional Assay
(1)
ICC
(1)
Immunoprecipitation
(1)
Neutralization
(4)
Western Blot
(1)
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Filter By Species
Human
(8)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB3291 Applications Species
Dorion, MF;Casas, D;Shlaifer, I;Yaqubi, M;Fleming, P;Karpilovsky, N;Chen, CX;Nicouleau, M;Piscopo, VEC;MacDougall, EJ;Alluli, A;Goldsmith, TM;Schneider, A;Dorion, S;Aprahamian, N;MacDonald, A;Thomas, RA;Dudley, RWR;Hall, JA;Fon, EA;Antel, JP;Stratton, JA;Durcan, TM;La Piana, R;Healy, LM; An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders Molecular neurodegeneration 2024-04-05 [PMID: 38576039] (Western Blot, Human) Western Blot Human
M Tkach, J Thalmensi, E Timperi, P Gueguen, N Névo, E Grisard, P Sirven, F Cocozza, A Gouronnec, L Martin-Jau, M Jouve, F Delisle, N Manel, DC Rookhuizen, CL Guerin, V Soumelis, E Romano, E Segura, C Théry Extracellular vesicles from triple negative breast cancer promote pro-inflammatory macrophages associated with better clinical outcome Proceedings of the National Academy of Sciences of the United States of America, 2022-04-19;119(17):e2107394119. 2022-04-19 [PMID: 35439048] (Functional Assay, Human) Functional Assay Human
C Emre, KV Do, B Jun, E Hjorth, SG Alcalde, MI Kautzmann, WC Gordon, P Nilsson, NG Bazan, M Schultzber Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer&#039;s disease Acta neuropathologica communications, 2021-06-29;9(1):116. 6/29/2021 [PMID: 34187579]
Xia Gao, Guiquan Jia, Anna Guttman, Daryle J. DePianto, Katrina B. Morshead, Kai-Hui Sun et al. Osteopontin Links Myeloid Activation and Disease Progression in Systemic Sclerosis Cell Reports Medicine 11/17/2020 [PMID: 33294861]
HH Woo, SC Lee, JB Stoffer, D Rush, SK Chambers Phenotype of vigilin expressing breast cancer cells binding to the 69 nt 3&#039;UTR element in CSF-1R mRNA Transl Oncol, 2018-10-03;12(1):106-115. 2018-10-03 [PMID: 30292064] (Bioassay, Human) Bioassay Human
B Wang, Z Ma, M Wang, X Sun, Y Tang, M Li, Y Zhang, F Li, X Li IL-34 Upregulated Th17 Production through Increased IL-6 Expression by Rheumatoid Fibroblast-Like Synoviocytes Mediators Inflamm., 2017-06-04;2017(0):1567120. 2017-06-04 [PMID: 28659662] (Neutralization, Human) Neutralization Human
Receptor-Type Protein-Tyrosine Phosphatase ? and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1 PLoS ONE, 2016-11-29;11(11):e0167324. 2016-11-29 [PMID: 27898738] (Neutralization, Human) Neutralization Human
Jeanine Pignatelli, Sumanta Goswami, Joan G. Jones, Thomas E. Rohan, Evan Pieri, Xiaoming Chen et al. Invasive breast carcinoma cells from patients exhibit Mena INV - and macrophage-dependent transendothelial migration Science Signaling 11/25/2014 [PMID: 25429076]
Patsialou A, Wang Y, Pignatelli J, Chen X, Entenberg D, Oktay M, Condeelis J Autocrine CSF1R signaling mediates switching between invasion and proliferation downstream of TGFbeta in claudin-low breast tumor cells. Oncogene, 2014-08-04;34(21):2721-31. 2014-08-04 [PMID: 25088194] (Neutralization, Human) Neutralization Human
Forget M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, Moldovan L, Mo X, Evans R, Marsh C, Eubank T Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer. PLoS ONE, 2014-06-03;9(6):e98623. 2014-06-03 [PMID: 24892425] (Neutralization, Human) Neutralization Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol CSF1R
Uniprot