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Recombinant Human M-CSF His Protein

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SDS-Page: Recombinant Human M-CSF His Protein [NBP1-30174] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
SDS-Page: Recombinant Human M-CSF His Protein [NBP1-30174] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human M-CSF His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 33-190 of Human M-CSF

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ DVVTKPDCN
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CSF1
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 2 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human M-CSF His Protein

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

Macrophage colony stimulating factor (M-CSF), as known as CSF-1, one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osteoclasts. Recombinant human M-CSF, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CSF1