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Rat M-CSF ELISA Kit (Colorimetric)

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ELISA: Rat M-CSF ELISA Kit (Colorimetric) [NBP3-18031] - Samples were spiked with high concentrations of Rat M-CSF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat M-CSF ELISA Kit (Colorimetric) [NBP3-18031] - Samples were spiked with high concentrations of Rat M-CSF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat M-CSF ELISA Kit (Colorimetric) [NBP3-18031] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

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Rat M-CSF ELISA Kit (Colorimetric) Summary

Standard Curve Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 6.17
Intra-Assay
%CV < 6.20
Spike Recovery
85-102%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CSF1

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat M-CSF ELISA Kit (Colorimetric)

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Product PDFs

  SDS
  Rat M-CSF ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol CSF1