Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Analysis of JMJD2C in HeLa nuclear extracts.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in ...read more
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Staining of JMJD2C in paraffin embedded mouse pancreas using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS ...read more
Simple Western: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Simple Western lane view shows a specific band for JMJD2C in 1.0 mg/ml of HeLa lysate. This experiment was performed under ...read more
Genetic Strategies: Knockdown Validated: Rabbit Polyclonal Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - shRNA Knockdown using 4C/KDM4C/JMJD2C Antibody. Image from a verified customer ...read more
Chromatin Immunoprecipitation reported in scientific literature (PMID 23884959; 23129632)
Immunocytochemistry/ Immunofluorescence 1:100
Immunohistochemistry 1:100
Immunohistochemistry-Paraffin 1:100
Immunoprecipitation reported in scientific literature (PMID 23129632)
Knockdown Validated Validated for Knockdown from a verified customer review.
Simple Western 1:12.5
Western Blot 1:1000
Application Notes
In Western blot, a band is seen ~119 kDa in HeLa cells. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nuclei of mouse pancreas. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
GASC1JmjC domain-containing histone demethylation protein 3C
Gene amplified in squamous cell carcinoma 1 protein
JHDM3C
JMJD2C
JMJD2CbA146B14.1
jumonji domain containing 2C
Jumonji domain-containing protein 2C
KDM4C
KIAA0780FLJ25949
Lysine (K)specific Demethylase 4C
Lysine (K)-specific Demethylase 4C
lysine-specific demethylase 4C
Background
JMJD2C (Jumonji C domain-containing oxygenase D2C; also called GASC1, JHDM3C or KDM4C) belongs to JMJD2 family and is a histone demethylase for di- and trimethylated lysine 9 and 36 on histone H3 (H3K9me3/2 and H3K36me3/2). H3K9me3/2 is generally associated with transcriptional repression and heterochromatin formation, whereas, H3K36me3/2 is linked to transcriptionaly active genes where it plays a key role in suppression of incorrect transcription. JMJD2C regulates the expression of pluripotency genes including NOTCH1, NANOG, Sox2 and Pou5, and because of its dual role in modifying H3 either by removing the repressive H3K9me3/2 or the active H3K36me3/2 factor, JMJD2C has been considered a fine-tuning regulator of gene expression in normal development/differentiation as well as in carcinogenic progression. JMJD2C is overexpressed in aggressive, basal-like breast cancers compared with non basal-like breast cancers, and its overexpression has been shown to cause transformation of immortalized, non-transformed mammary epithelial cells and to regulate expression of genes responsible for stem cell phenotype/self-renewal in breast cancer cells. Moreover oncogenic role of JMJD2C has been documented in prostate cancer where it enhances the transcription of AR-dependent genes and cell proliferation by interaction with ligand-bound AR.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (NBP1-49600). (Showing 1 - 2 of 2 FAQs).
I am interested in the KDM4cJMJD2c antibody NBP1-49600. According to their website, it is used against human and murine JMJD2c protein. In addition, there is an image of an Immunhisto (Mouse pancreas).We need the antibody for the main part for Western blots with mouse protein. Are there positive reviews about it? Did they specify the reactivity? How specific does it recognize the protein? Does it recognize the protein properly? For an antibody of a different company, we already had the problem that mouse was specified on their datasheet but in the end wasn't recognized.
This antibody, being a polyclonal does recognize both the human and mouse proteins. It has been shown to work much better on the native mouse proteins in IHC than in Western blot. It does recognize mouse JMJD2C in Western blot but we have seen that it does give some background staining. Regardless, we have a 100% guarantee on all of our products for the applications and species listed on our datasheet, so we will guarantee this for Western blot on mouse samples.
How has this antibody been tested for reactivity with mouse other than immunohistochemistry?
We do not have data for NBP1-49600 with mouse other than in IHC-P. However, if you use NBP1-49600 to analyze your mouse samples in any of the validated applications (those stated on the datasheet) for this antibody, you would be covered by our Novus Guarantee, which means that if you did have any issues, we would resolve them.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Analysis of JMJD2C in HeLa nuclear extracts.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-Western-Blot-NBP1-49600-img0006.jpg "Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Analysis of JMJD2C in HeLa nuclear extracts.")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody NBP1-49600 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49600-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody NBP1-49600 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Staining of JMJD2C in paraffin embedded mouse pancreas using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-Immunohistochemistry-Paraffin-NBP1-49600-img0005.jpg "Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Staining of JMJD2C in paraffin embedded mouse pancreas using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody NBP1-49600 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49600-img0008.jpg "Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody NBP1-49600 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Simple Western: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Simple Western lane view shows a specific band for JMJD2C in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4C-KDM4C-JMJD2C-Antibody-Simple-Western-NBP1-49600-img0007.jpg "Simple Western: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Simple Western lane view shows a specific band for JMJD2C in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Knockdown Validated: Rabbit Polyclonal Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - shRNA Knockdown using 4C/KDM4C/JMJD2C Antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-49600_rabbit-polyclonal-lysine-k-specific-demethylase-4c-kdm4c-jmjd2c-antibody-knockdown-validated-17102024104813..png "Knockdown Validated: Rabbit Polyclonal Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - shRNA Knockdown using 4C/KDM4C/JMJD2C Antibody. Image from a verified customer review.")
![Western Blot G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241252118.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-412024125214.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241251265.jpg)
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![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
