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Lyn Antibody (338716) [Unconjugated]

 
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Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Lyn at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # ...read more
Lyn was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206) at 25 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyn at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Rat ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
338716
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3206-SP
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Lyn Antibody (338716) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Lyn
Met1-Ile67
Accession # P07948
Specificity
Detects human and rat Lyn.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LYN
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lyn Antibody (338716) [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ26625
  • Hck-2
  • JTK8
  • Lyn
  • tyrosine-protein kinase Lyn
  • v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
  • v-yes-1
  • Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

The nonreceptor tyrosine kinase Lyn (lck/yes-related novel tyrosine kinase) is widely expressed, and the predominant Src family member present in B cells. Activation of Lyn occurs upon its association with cell surface receptors such as BCR and CD40. Analyses from both loss- and gain-of-function mutant mice have revealed that Lyn is an essential regulator of B cell activation, maturation, and tolerance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lyn Antibody
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Bioinformatics

Gene Symbol LYN
Uniprot