Novus Biologicals products are now on bio-techne.com

LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free

Images

 
Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 69-329 of human NR1H3 (NP_005684) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NR1H3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free

  • alpha
  • Liver X receptor alpha
  • liver X receptor-alpha
  • LXR alpha
  • LXRA
  • NR1H3
  • Nuclear receptor subfamily 1 group H member 3
  • nuclear receptor subfamily 1, group H, member 3
  • oxysterols receptor LXR-alpha
  • RLD-1

Background

Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of cholesterol metabolism and bile acids. Two known isoforms, alpha and beta, are differentially expressed. The alpha isoform is predominantly expressed in liver, whereas the beta isoform expression has been shown to be ubiquitous.

LXR antibodies are useful for lipid and cholesterol metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-74457
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
PP-A9033A-00
Species: Hu
Applications: DirELISA, IHC, IP, WB
NB400-105
Species: Ca, Ch, ChHa, Eq, Ha, Hu, Mu, Md, Po, Pm, Rb, Rt
Applications: B/N, ChIP, ChIP, Dual ISH-IHC, ELISA, Flow, GS, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PCR, Simple Western, WB
PP-H4417-00
Species: Hu
Applications: DirELISA, IP, WB
NB300-537
Species: Bv, Hu, Mu, Rb, Rt
Applications: ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, KD, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
PP-N4111-00
Species: Hu
Applications: IHC, IP, WB
NBP2-22106
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-66778
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
NB600-582
Species: Ca, Ch, SyHa, Ha, Hu, Pm, Mu, Rt
Applications: IHC-Fr, KO, Simple Western, WB
NB400-114
Species: Ch, Fe, Ha, Hu, Mu, Pl, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
H00001594-Q01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-52813
Species: Hu, Rt
Applications: ICC/IF, IHC-P, WB
NLS5411
Species: Ha, Hu, Pm, Mu, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-72550
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for LXR alpha/NR1H3 Antibody (NBP2-72550) (0)

There are no publications for LXR alpha/NR1H3 Antibody (NBP2-72550).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LXR alpha/NR1H3 Antibody (NBP2-72550) (0)

There are no reviews for LXR alpha/NR1H3 Antibody (NBP2-72550). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LXR alpha/NR1H3 Antibody (NBP2-72550) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional LXR alpha/NR1H3 Products

Research Areas for LXR alpha/NR1H3 Antibody (NBP2-72550)

Find related products by research area.

Blogs on LXR alpha/NR1H3

There are no specific blogs for LXR alpha/NR1H3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol NR1H3