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LXR alpha/NR1H3 Antibody (OTI1A5)

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Western Blot: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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LXR alpha/NR1H3 Antibody (OTI1A5) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 69-329 of human NR1H3 (NP_005684) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NR1H3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-46220 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LXR alpha/NR1H3 Antibody (OTI1A5)

  • alpha
  • Liver X receptor alpha
  • liver X receptor-alpha
  • LXR alpha
  • LXRA
  • NR1H3
  • Nuclear receptor subfamily 1 group H member 3
  • nuclear receptor subfamily 1, group H, member 3
  • oxysterols receptor LXR-alpha
  • RLD-1

Background

Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of cholesterol metabolism and bile acids. Two known isoforms, alpha and beta, are differentially expressed. The alpha isoform is predominantly expressed in liver, whereas the beta isoform expression has been shown to be ubiquitous.

LXR antibodies are useful for lipid and cholesterol metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LXR alpha/NR1H3 Antibody (NBP2-46220)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, IF/IHC.


Filter By Application
Flow Cytometry
(1)
IF/IHC
(1)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP2-46220 Applications Species
Van Dongen K, Leleu D, Pilot T et al. Atheroma plaque microenvironment stimulates kynurenine production by macrophages to induce endothelial adhesion molecules in the context of atherogenesis bioRxiv 2023-07-24 (Flow Cytometry, Human)

Details:
1:200 Flow dilution
Flow Cytometry Human
MEnEgaut L, Thomas C, Jalil A et al. Interplay between Liver X Receptor and Hypoxia Inducible Factor 1 alpha Potentiates Interleukin-1 beta Production in Human Macrophages Cell Rep 2020-01-01 [PMID: 32433974] (IF/IHC, Human) IF/IHC Human

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NR1H3