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LLGL2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: LLGL2 Antibody [NBP1-81753] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LLGL2 Antibody [NBP1-81753] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: LLGL2 Antibody [NBP1-81753] - Staining of human fallopian tube shows moderate to strong cytoplasmic postivity in glandular cells.
Immunohistochemistry-Paraffin: LLGL2 Antibody [NBP1-81753] - Staining of human stomach shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: LLGL2 Antibody [NBP1-81753] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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LLGL2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TKYGQGFYLISPSEFERFSLSTKWLVEPRCLVDSAETKNHRPGNGAGPKKAPSRARNSGTQSDGEEKQPGLVMERALLSDERVLKEIQSTLEGDRGSGNWRSHRAAVGCSLSNGGAE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LLGL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
LLGL2 Protein (NBP1-81753PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LLGL2 Antibody

  • HGLlethal giant larvae (Drosophila) homolog 2
  • human giant larvae homolog
  • lethal giant larvae homolog 2 (Drosophila)
  • lethal(2) giant larvae protein homolog 2
  • LGL2

Background

The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-81753

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Bioinformatics

Gene Symbol LLGL2