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Recombinant Mouse LIGHT/TNFSF14 Protein

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SDS-Page: Recombinant Mouse LIGHT/TNFSF14 Protein [NBP2-61332]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Bioactivity
Concentration
LYOPH

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Recombinant Mouse LIGHT/TNFSF14 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 168 amino acids corresponding to LIGHT/TNFSF14 Source: E. coli

Amino Acid Sequence: DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV

Details of Functionality
LIGHT/TNFSF14 protein is fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using human HT-29 cells is less than 2 ug/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-g.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNFSF14
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of LIGHT/TNFSF14 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Western Blot
Theoretical MW
18.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Mouse LIGHT/TNFSF14 Protein

  • CD258 antigen
  • CD258
  • delta transmembrane LIGHT
  • Herpes virus entry mediator ligand
  • Herpesvirus entry mediator ligand
  • HVEM-L
  • HVEMLherpesvirus entry mediator-ligand
  • ligand for herpesvirus entry mediator
  • LIGHT
  • LIGHTherpesvirus entry mediator A
  • LTg
  • TNFSF14
  • TR2
  • tumor necrosis factor (ligand) superfamily, member 14
  • tumor necrosis factor ligand superfamily member 14
  • tumor necrosis factor receptor-like 2
  • tumor necrosis factor superfamily member LIGHT

Background

TNFSF14 is encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TNFSF14