Mouse Leptin R ELISA Kit (Colorimetric) Summary
Standard Curve Range |
3.13 - 200 ng/mL |
Sensitivity |
1.88 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
%CV < 5.21 |
Intra-Assay |
%CV < 5.64 |
Spike Recovery |
93 - 109% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
LEPR |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Antibody Diluent
- Concentrated Biotinylated Detection Antibody
- Concentrated HRP Conjugate
- Concentrated Wash Buffer
- HRP Conjugate Diluent
- Micro ELISA Plate
- Plate Sealer
- Reference Standard & Sample Diluent
- Reference Standard
- Stop Solution
- Substrate Reagent
|
Alternate Names for Mouse Leptin R ELISA Kit (Colorimetric)
Background
Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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