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Mouse Leptin R ELISA Kit (Colorimetric)

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ELISA: Mouse Leptin R ELISA Kit (Colorimetric) [NBP3-11805] - Samples were spiked with high concentrations of Mouse Leptin R and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Mouse Leptin R ELISA Kit (Colorimetric) [NBP3-11805] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL

Order Details

Mouse Leptin R ELISA Kit (Colorimetric) Summary

Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.21
Intra-Assay
%CV < 5.64
Spike Recovery
93 - 109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LEPR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Antibody Diluent
  2. Concentrated Biotinylated Detection Antibody
  3. Concentrated HRP Conjugate
  4. Concentrated Wash Buffer
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate
  7. Plate Sealer
  8. Reference Standard & Sample Diluent
  9. Reference Standard
  10. Stop Solution
  11. Substrate Reagent

Alternate Names for Mouse Leptin R ELISA Kit (Colorimetric)

  • B219
  • CD295 antigen
  • CD295
  • DB
  • DKFZp686B1731
  • huB219
  • LEPR
  • LEP-R
  • Leptin R
  • leptin receptor
  • LeptinR
  • OB R
  • OB receptor
  • OB-R
  • OBRCD295

Background

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LEPR