Recombinant Human Leptin/OB Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Human Leptin plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Ob (Lep) gene (Ob for obese, Lep for leptin) is located on chromosome 7 in humans. The protein is manufactured primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. Leptin acts on receptors in the hypothalamus of the brain where it inhibits appetite by (1) counteracting the effects of neuropeptide Y (a potent feeding stimulant secreted by cells in the gut and in the hypothalamus); (2) counteracting the effects of anandamide (another potent feeding stimulant that binds to the same receptors as THC), and (3) promoting the synthesis of alpha-MSH, an appetite suppressant. This appetite inhibition is long-term, in contrast to the rapid inhibition of eating by cholecystokinin (CCK) and the slower suppression of hunger between meals mediated by PYY3- 36. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publication using NBP2-34934	Applications	Species
Curtis KJ, Mai C, Martin H et al. The Effect of Marrow Secretome and Culture Environment on the Rate of Metastatic Breast Cancer Cell Migration in Two and Three Dimensions Molecular biology of the cell 2021-03-10 [PMID: 33689396]

Array NBP2-34934

• Leptin/OB Antibodies
• Leptin/OB Antibody Pair
• Leptin/OB ELISA Kit
• Leptin/OB Lysate
• Leptin/OB Protein
• Leptin/OB ELISAs
• Leptin/OB Proteins and Enzymes