Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric) Summary
Description |
Quality control test: Standard curve. Lep (Mouse/Rat) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse and/or rat leptin in serum, plasma. |
Standard Curve Range |
100 to 4000 pg/mL |
Sensitivity |
30 pg/mL (mouse), 50 pg/mL (rat) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 3.40 |
Intra-Assay |
%CV < 2.20 |
Spike Recovery |
94.50% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
LEP |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
|
- Antibody Coated Microtiter Strips (Ready to use)
- Biotin Labelled Antibody Conc.(10x)
- Biotin–Ab Diluent (Ready to use)
- Dilution Buffer (Ready to use)
- Master Standard Mouse (lyophilized)
- Master Standard Rat (lyophilized)
- Quality Control Mouse (lyophilized)
- Quality Control Rat (lyophilized)
- Stop Solution (Ready to use)
- Streptavidin-HRP Conjugate (Ready to use)
- Substrate Solution (Ready to use)
- Wash Solution Conc. (10x)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human, Mouse, Rat Leptin/OB ELISA Kit (Colorimetric)
Background
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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