LDLR Antibody (C7) - Azide and BSA Free Summary
Immunogen |
Partially purified bovine LDL receptor [Swiss-Prot# P01131]. |
Localization |
Cell membrane/cell surface, endomembrane system, endosomes, lysosome, clathrin-coated pit, and Golgi apparatus |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
LDLR |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Electron Microscopy reported in scientific literature (PMID 11839845)
- ELISA
- Flow Cytometry reported in scientific literature (PMID 10906332)
- Immunocytochemistry/ Immunofluorescence 1:100
- Immunohistochemistry 1:200
- Immunohistochemistry-Frozen reported in scientific literature (PMID 11839845)
- Immunohistochemistry-Paraffin 1:200
- Immunoprecipitation 1:10 - 1:500
- In vitro assay reported in scientific literature (PMID 9642270)
- Radioimmunoassay
- Western Blot 1:1000
|
Application Notes |
In WB assay, specific bands can be seen around 160 kDa (mature form) and 120 kDa (precursor) molecular weight positions. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
Tris-Glycine, 0.15M NaCl |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein G purified |
Alternate Names for LDLR Antibody (C7) - Azide and BSA Free
Background
LDL Receptor (low-density lipoprotein receptor or LDLR) is a key determinant of plasma cholesterol levels and as a ubiquitously expressed cell membrane glycoprotein, it binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells via endocytosis. LDLR is found distributed from plasma membrane to intracellular compartments, cell surface (in the presence of PCSK9) and also localizes to Golgi apparatus as well as early/late endosomes and lysosomes. VLDL, IDL, HDL, and chylomicron remnant are also recognized by LDLR at neutral pH. Receptor-ligand complexes undergo endocytosis via clathrin-coated pits and coated vehicle dispenses to endosomes with LRP6 and ARH (also known as LDLR adaptor protein), connecting LDLR family protein and the endocytic machinery; thereby, acidic condition activates dissociation of internalized ligands. Released ligand particles further travel to lysosomes wherein the ligand is degraded by enzyme, while the receptors recycle back to cell surface. After internalization, LDL particles trigger reduction in HMGCR expression to suppress cholesterol biosynthesis; enhancement of ACAT activity to reduce toxic free cholesterol, and suppression of LDLR synthesis to reduce LDL uptake via SREBPs. Genetic mutations impairing LDLR function results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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