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LDLR Antibody (C7) - Azide and BSA Free

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Western Blot: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2. Image from the standard format of this ...read more
Immunohistochemistry-Paraffin: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin ...read more
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Antibody was tested in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue). Image from the standard ...read more

Product Details

Summary
Reactivity Hu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC, IP, MS
Clone
C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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LDLR Antibody (C7) - Azide and BSA Free Summary

Immunogen
Partially purified bovine LDL receptor [Swiss-Prot# P01131].
Localization
Cell membrane/cell surface, endomembrane system, endosomes, lysosome, clathrin-coated pit, and Golgi apparatus
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LDLR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy reported in scientific literature (PMID 11839845)
  • ELISA
  • Flow Cytometry reported in scientific literature (PMID 10906332)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 11839845)
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay reported in scientific literature (PMID 9642270)
  • Radioimmunoassay
  • Western Blot 1:1000
Application Notes
In WB assay, specific bands can be seen around 160 kDa (mature form) and 120 kDa (precursor) molecular weight positions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for LDLR Antibody (C7) - Azide and BSA Free

  • FH
  • FHC
  • LDL R
  • LDL receptor
  • LDLCQ2
  • LDLR
  • low density lipoprotein receptor
  • low-density lipoprotein receptor class A domain-containing protein 3
  • low-density lipoprotein receptor

Background

LDL Receptor (low-density lipoprotein receptor or LDLR) is a key determinant of plasma cholesterol levels and as a ubiquitously expressed cell membrane glycoprotein, it binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells via endocytosis. LDLR is found distributed from plasma membrane to intracellular compartments, cell surface (in the presence of PCSK9) and also localizes to Golgi apparatus as well as early/late endosomes and lysosomes. VLDL, IDL, HDL, and chylomicron remnant are also recognized by LDLR at neutral pH. Receptor-ligand complexes undergo endocytosis via clathrin-coated pits and coated vehicle dispenses to endosomes with LRP6 and ARH (also known as LDLR adaptor protein), connecting LDLR family protein and the endocytic machinery; thereby, acidic condition activates dissociation of internalized ligands. Released ligand particles further travel to lysosomes wherein the ligand is degraded by enzyme, while the receptors recycle back to cell surface. After internalization, LDL particles trigger reduction in HMGCR expression to suppress cholesterol biosynthesis; enhancement of ACAT activity to reduce toxic free cholesterol, and suppression of LDLR synthesis to reduce LDL uptake via SREBPs. Genetic mutations impairing LDLR function results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LDLR