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Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]

 (7 Publications)
 
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Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB4124) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB4124) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more
Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB4124) at 5 µg/mL for 1 hour at room ...read more
Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B/ApoB at approximately 333 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
369717
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB4124-SP
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Apolipoprotein B/ApoB Antibody (369717) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ApoB
Met1206-Asp1413
Accession # NP_000375
Specificity
Detects human Apolipoprotein B in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APOB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB4124 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]

  • Apo B-100
  • APOB
  • ApoB-100
  • ApoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100

Background

Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 amino acids (aa) in length. It binds LDL to the ApoB/E receptor. Multiple forms of ApoB-100 exist; ApoB-25, 26, 29, 37, 39, 48, and 74. The numbers reflect each molecule’s predicted MW as a percentage of ApoB-100 (512 kDa). Amino acids 1206-1414 of the precursor were used for immunization. Based on likelihood of secretion, this antibody may recognize B26 (1297 aa), B37 (1728 aa), B39 (1799 aa), and B48 (2152 aa). Over the immunization polypeptide range, human ApoB-100 is 80% and 82% aa identical to mouse and dog ApoB-100, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]
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Publications for Apolipoprotein B/ApoB Antibody (MAB4124)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


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Flow Cytometry
(1)
Western Blot
(2)
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(3)
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Showing Publications 1 - 7 of 7.
Publications using MAB4124 Applications Species
Dmitry Ter-Ovanesyan, Tal Gilboa, Bogdan Budnik, Adele Nikitina, Sara Whiteman, Roey Lazarovits et al. Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins eLife 2023-05-30 [PMID: 37252755]
JD Spitzberg, S Ferguson, KS Yang, HM Peterson, JCT Carlson, R Weissleder Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact Nature Communications, 2023;14(1):1239. 2023 [PMID: 36870999] (Western Blot, Human) Western Blot Human
JD Spitzberg, S Ferguson, KS Yang, HM Peterson, JCT Carlson, R Weissleder Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact Nature Communications, 2023-03-04;14(1):1239. 2023-03-04 [PMID: 36870999] (Western Blot, Human) Western Blot Human
Christian A. Boada, Assaf Zinger, Scott Rohen, Jonathan O. Martinez, Michael Evangelopoulos, Roberto Molinaro et al. LDL-Based Lipid Nanoparticle Derived for Blood Plasma Accumulates Preferentially in Atherosclerotic Plaque Frontiers in Bioengineering and Biotechnology 2021-12-01 [PMID: 34926432]
Megan I. Mitchell, Iddo Z. Ben‐Dov, Christina Liu, Kenny Ye, Kar Chow, Yael Kramer et al. Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV‐CATCHER): A customizable purification assay designed for small‐RNA biomarker identification and evaluation of circulating small‐EVs Journal of Extracellular Vesicles 2021-06-03 [PMID: 34122779]
K Kim, IJ Goldberg, MJ Graham, M Sundaram, E Bertaggia, SX Lee, L Qiang, RA Haeusler, D Metzger, P Chambon, Z Yao, HN Ginsberg, UB Pajvani ?-Secretase Inhibition Lowers Plasma Triglyceride-Rich Lipoproteins by Stabilizing the LDL Receptor Cell Metab., 2018-03-22;0(0):. 3/22/2018 [PMID: 29576536]
Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology. J. Thromb. Haemost., 2011-05-01;9(5):1056-66. 2011-05-01 [PMID: 21342432] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol APOB
Uniprot