LDLR Antibody (C7)

LDL Receptor (low-density lipoprotein receptor or LDLR) is a key determinant of plasma cholesterol levels and as a ubiquitously expressed cell membrane glycoprotein, it binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells via endocytosis. LDLR is found distributed from plasma membrane to intracellular compartments, cell surface (in the presence of PCSK9) and also localizes to Golgi apparatus as well as early/late endosomes and lysosomes. VLDL, IDL, HDL, and chylomicron remnant are also recognized by LDLR at neutral pH. Receptor-ligand complexes undergo endocytosis via clathrin-coated pits and coated vehicle dispenses to endosomes with LRP6 and ARH (also known as LDLR adaptor protein), connecting LDLR family protein and the endocytic machinery; thereby, acidic condition activates dissociation of internalized ligands. Released ligand particles further travel to lysosomes wherein the ligand is degraded by enzyme, while the receptors recycle back to cell surface. After internalization, LDL particles trigger reduction in HMGCR expression to suppress cholesterol biosynthesis; enhancement of ACAT activity to reduce toxic free cholesterol, and suppression of LDLR synthesis to reduce LDL uptake via SREBPs. Genetic mutations impairing LDLR function results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Porcine.

We have publications tested in 9 applications: ELISA, EM, FLOW, ICC/IF, IHC-Fr, IP, In vitro, RIA, WB.

Showing Publications 1 - 10 of 22. Show All 22 Publications. Collapse Publications.

Publications using NBP1-78159	Applications	Species
Lee E, Jung Y, Park Y et al. A distinct astrocyte subtype in the aging mouse brain characterized by impaired protein homeostasis Nature Aging 2022-08-01 [PMID: 37118130] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Anthony H, Thomas O, Martin C et al. Cholesterol supports bovine granulosa cell inflammatory responses to lipopolysaccharide Society for Reproduction and Fertility 2022-08-05 [PMID: 35900358] (WB, Bovine)	WB	Bovine
Riad A, Lengyel-Zhand Z, Zeng C et Al. The Sigma-2 Receptor/TMEM97, PGRMC1, and LDL Receptor complex are responsible for the cellular uptake of A beta 42 and its protein aggregates Mol Neurobiol 2020-06-24 [PMID: 32572762]
Omer L, Hindi L, Militello G et al. Familial hypercholesterolemia class II low density lipoprotein-receptor response to statin treatment Dis Model Mech 2020-01-31 [PMID: 32005714] (FLOW, Human)	FLOW	Human
Riad A, Zeng C, Weng CC et al. Sigma-2 Receptor/TMEM97 and PGRMC-1 Increase the Rate of Internalization of LDL by LDL Receptor through the Formation of a Ternary Complex. Sci Rep. 2018-11-15 [PMID: 30443021] (ICC/IF, Human)	ICC/IF	Human
Sato M, Miyoshi K, Nakamura S et al. Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System. Int J Mol Sci 2017-12-04 [PMID: 29207527] (Porcine)		Porcine
Chen Hc, Chen Py, Wu Mj et al. Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells. PLoS ONE 2016-09-13 [PMID: 27617748] (WB)	WB
Granato M, Zompetta C, Vescarelli E et al. HCV derived from sera of HCV-infected patients induces pro-fibrotic effects in human primary fibroblasts by activating GLI2. Sci Rep. 2016-08-01 [PMID: 27476557] (WB, Human)	WB	Human
Pena F, Jansens A, van Zadelhoff G, Braakman I. Calcium as a crucial cofactor for low density lipoprotein receptor folding in the endoplasmic reticulum. J Biol Chem. 2010-03-19 [PMID: 20089850]
Roosendaal SD, Kerver J, Schipper M et al. The complex of the insect LDL receptor homolog, lipophorin receptor, LpR, and its lipoprotein ligand does not dissociate under endosomal conditions. FEBS J. 2008-04-01 [PMID: 18331356] (FLOW)	FLOW
Blasiole DA, Oler AT, Attie AD. Regulation of ApoB secretion by the low density lipoprotein receptor requires exit from the endoplasmic reticulum and interaction with ApoE or ApoB. J Biol Chem. 2008-04-25 [PMID: 18272520] (ICC/IF)	ICC/IF
Nguyen AT, Hirama T, Chauhan V et al. Binding characteristics of a panel of monoclonal antibodies against the ligand binding domain of the human LDLr. J Lipid Res. 2006-07-01 [PMID: 16601300]
Maxwell KN, Fisher EA, Breslow JL. Overexpression of PCSK9 accelerates the degradation of the LDLR in a post-endoplasmic reticulum compartment. Proc Natl Acad Sci U S A. 102(6):2069-74. 2005-02-08 [PMID: 15677715] (IP, Human)	IP	Human
Gillian-Daniel DL, Bates PW, Tebon A, Attie AD. Endoplasmic reticulum localization of the low density lipoprotein receptor mediates presecretory degradation of apolipoprotein B. Proc Natl Acad Sci U S A. 99(7):4337-42. 2002-04-02 [PMID: 11904390] (ICC/IF, Human)	ICC/IF	Human
Jaworska-Wilczynska M, Wilczynski GM, Engel WK et al. Three lipoprotein receptors and cholesterol in inclusion-body myositis muscle. Neurology. 2002-02-12 [PMID: 11839845] (EM, IHC-Fr, Human)	EM, IHC-Fr	Human
Jorgensen MM1, Jensen ON, Holst HU et al. Grp78 is involved in retention of mutant low density lipoprotein receptor protein in the endoplasmic reticulum. J Biol Chem. 2000-10-27 [PMID: 10906332] (IP, FLOW, Human)	IP, FLOW	Human
Warren RA, Green FA, Stenberg PE, Enns CA. Distinct saturable pathways for the endocytosis of different tyrosine motifs. J Biol Chem. 1998-07-03 [PMID: 9642270] (In vitro)	In vitro
Hofer F, Gruenberger M, Kowalski H, Machat H, Huettinger M, Kuechler E, Blaas D. Members of the low density lipoprotein receptor family mediate cell entry of a minor-group common cold virus. Proc Natl Acad Sci U S A. 91(5):1839-42. 1994-03-01 [PMID: 8127891] (ELISA, WB, Human)	ELISA, WB	Human
Jensen TG, Roses AD, Jorgensen AL. Apolipoprotein E uptake and degradation via chloroquine-sensitive pathway in cultivated monkey cells overexpressing low density lipoprotein receptor. Neurosci Lett. 1994-10-24 [PMID: 7700578] (ICC/IF, Human) Details: LDLR antibody (clone C7) was used for immunostaining of COS-7 cells (an SV40 transformed Simian kidney cell line) which was transiently transfected with human low density lipoprotein receptor (LDLR).	ICC/IF	Human
Somia NV, Zoppe M, Verma IM. Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery. Proc Natl Acad Sci U S A. 92(16):7570-4. 1995-08-01 [PMID: 7638232] (WB, Human)	WB	Human
Beisiegel U, Schneider WJ, Goldstein JL, Anderson RG, Brown MS. Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J Biol Chem. 256(22):11923-31. 1981-11-25 [PMID: 6271765] (ICC/IF, RIA, Human)	ICC/IF, RIA	Human
Leitersdorf E, Hobbs HH, Fourie AM, Jacobs M, van der Westhuyzen DR, Coetzee GA. Deletion in the first cysteine-rich repeat of low density lipoprotein receptor impairs its transport but not lipoprotein binding in fibroblasts from a subject with familial hypercholesterolemia. Proc Natl Acad Sci U S A. 85(21):7912-6. 1988-11-01 [PMID: 3263645] (IP, RIA, Human)	IP, RIA	Human
Show All 22 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Kwonil Jung	ICC	Other	12/03/2013	View Summary Application Immunocytochemistry Sample Tested A porcine intestinal epithelial cell line, named as IPEC-J2 Species Other

Array NBP1-78159

• LDLR Antibodies
• LDLR Antibody Pair
• LDLR ELISA Kit
• LDLR Lysate
• LDLR Proteins
• LDLR Proteins and Enzymes
• LDLR ELISAs