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LAP (TGF-beta 1) Antibody [Unconjugated]

 (11 Publications)
 (1 Review)
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TGF-beta 1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
TGF-beta 1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA) at 10 µg/mL for 3 hours at room temperature. ...read more
Recombinant Human LAP TGF‑ beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF‑ beta 1 (Catalog # 240‑B) growth inhibition activity in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). ...read more
LAP (TGF-beta 1) was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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LAP (TGF-beta 1) Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF‑ beta 1) and Chinese hamster ovary cell line CHO-derived recombinant human LAP (TGF‑ beta 1)
Leu30-Ser390
Accession # P01137
Specificity
Detects human LAP in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody is specific for the LAP derived from the TGF-beta 1 precursor but does not react with LAP from the TGF-beta 2 precursor.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFB1
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 15-30 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AB-246-NA in the following applications:
Publications
Read Publications using
AB-246-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAP (TGF-beta 1) Antibody [Unconjugated]

  • CED
  • DPD1
  • LAP (TGFbeta 1)
  • LAP (TGF-beta 1)
  • LAP
  • TGFB
  • TGFB1
  • TGFbeta
  • transforming growth factor beta 1

Background

TGF-  beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta  superfamily. TGF-  beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N-terminal 249 aa (aa 30-278) latency-associated peptide (LAP) and a C-terminal 112 aa (aa 279-390) mature TGF-  beta 1 (4‑6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (4‑8). Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5‑7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1 (10).

  1. Dunker, N. & K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  2. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  3. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  4. Derynck, R. et al. (1985) Nature 316:701.
  5. Dabovic, B. and D.B. Rifkin (2008) “TGF-beta Bioavailability” in The TGF-beta Family. Derynck, R. and K. Miyazono (eds): Cold Spring Harbor Laboratory Press, p. 179.
  6. Brunner, A.M. et al. (1989) J. Biol. Chem. 264:13660.
  7. Miyazono, K. et al. (1991) EMBO J. 10:1091.
  8. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  9. Miller, D.M. et al. (1992) Mol. Endocrinol. 6:694.
  10. Janssens, K. et al. (2003) J. Biol. Chem. 278:7718.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAP (TGF-beta 1) Antibody (AB-246-NA)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(3)
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Filter By Species
Human
(3)
Mouse
(2)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AB-246-NA Applications Species
Oezel, L;Wohltmann, M;Gondorf, N;Wille, J;Güven, I;Windolf, J;Thelen, S;Jaekel, C;Grotheer, V; Dupuytren's Disease Is Mediated by Insufficient TGF-?1 Release and Degradation International journal of molecular sciences 2023-10-11 [PMID: 37894778] (Western Blot, Human) Western Blot Human
Juo-Chin Yao, Karolyn A. Oetjen, Tianjiao Wang, Haoliang Xu, Grazia Abou-Ezzi, Joseph R. Krambs et al. TGF-beta signaling in myeloproliferative neoplasms contributes to myelofibrosis without disrupting the hematopoietic niche Journal of Clinical Investigation 6/1/2022 [PMID: 35439167]
Nagako Yoshiba, Kunihiko Yoshiba, Naoto Ohkura, Erika Takei, Naoki Edanami, Youhei Oda et al. Correlation between Fibrillin-1 Degradation and mRNA Downregulation and Myofibroblast Differentiation in Cultured Human Dental Pulp Tissue Journal of Histochemistry & Cytochemistry 6/1/2015 [PMID: 25805839]
Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment. Sci Rep, 2015-03-09;5(0):8919. 2015-03-09 [PMID: 25747469] (IHC, Mouse) IHC Mouse
Wottawa M, Leisering P, Ahlen M, Schnelle M, Vogel S, Malz C, Bordoli M, Camenisch G, Hesse A, Napp J, Alves F, Kristiansen G, Farhat K, Katschinski D Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-beta1 processing. Int J Cancer, 2012-12-27;132(12):2787-98. 2012-12-27 [PMID: 23225569] (Western Blot, Human) Western Blot Human
Lauzier A, Charbonneau M, Paquette M, Harper K, Dubois CM Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis. Arthritis Res. Ther., 2012-07-04;14(4):R159. 2012-07-04 [PMID: 22762273] (Western Blot, Human) Western Blot Human
V. Singh, V. Agrawal, M.R. Santhiago, S.E. Wilson Stromal fibroblast–bone marrow-derived cell interactions: Implications for myofibroblast development in the cornea Experimental Eye Research 5/1/2012 [PMID: 22465408]
Javier Milara, Adela Serrano, Teresa Peiró, Amadeu Gavaldà, Montserrat Miralpeix, Esteban Jesús Morcillo et al. Aclidinium inhibits human lung fibroblast to myofibroblast transition Thorax 3/1/2012 [PMID: 21957094]
Dana A Massuto, Eric C Kneese, Gregory A Johnson, Robert C Burghardt, R Neil Hooper, Nancy H Ing et al. Transforming growth factor beta (TGFB) signaling is activated during porcine implantation: proposed role for latency-associated peptide interactions with integrins at the conceptus–maternal interface REPRODUCTION 2/1/2010 [PMID: 19920116]
Dasgupta S, Bhattacharya-Chatterjee M, O&amp;apos;Malley BW, Chatterjee SK Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells. J. Cell. Biochem., 2006-04-01;97(5):1036-51. 2006-04-01 [PMID: 16294321] (IHC-Fr, IHC-P, Mouse) IHC-Fr, IHC-P Mouse
Show All 11 Publications.

Review for LAP (TGF-beta 1) Antibody (AB-246-NA) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AB-246-NA:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Human 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22334695
SpeciesHuman

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Bioinformatics

Gene Symbol TGFB1
Uniprot