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Lamin A + C Antibody (4L8Q0)

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Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of various cell lines, using Lamin A + C antibody (NBP3-15754) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of U2OS cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100 - 1:1600. Blue: DAPI for ...read more
Immunohistochemistry-Paraffin: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A + C antibody (NBP3-15754) at dilution of 1:400 - 1:3200 (40x ...read more
Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit ...read more
Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of various cell lines, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of H9C2 cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:200. Blue: DAPI for nuclear ...read more
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of L929 cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:200. Blue: DAPI for nuclear ...read more
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Confocal immunofluorescence analysis of HeLa cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100. Blue: DAPI for ...read more
Immunohistochemistry-Paraffin: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A + C antibody (NBP3-15754) at dilution of 1:200 (40x lens).Perform ...read more
Genetic Strategies: Knockout Validated: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts from normal (control) and Lamin A + C knockout (KO) HeLa cells, using Lamin A + C antibody ...read more
Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A + C antibody (NBP3-15754). Western blot was performed from the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KO
Clone
4L8Q0
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Lamin A + C Antibody (4L8Q0) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A + C (NP_733821.1). SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSS
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
LMNA
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:400
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • Knockout Validated
  • Western Blot 1:10000 - 1:600000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for Lamin A + C Antibody (4L8Q0)

  • CDCD1
  • CDDC
  • CMD1A
  • CMT2B1
  • dilated 1A (autosomal dominant)
  • EMD2
  • FPLD
  • HGPSFPL
  • lamin A/C
  • lamin A/C-like 1
  • lamin-A/C
  • LDP1
  • LFP
  • LGMD1B
  • limb girdle muscular dystrophy 1B (autosomal dominant)
  • LMN1IDC
  • LMNC
  • LMNL1
  • prelamin-A/C
  • PRO1,70 kDa lamin
  • progeria 1 (Hutchinson-Gilford type)
  • renal carcinoma antigen NY-REN-32

Background

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LMNA