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Lamin B1 Antibody (CL3929)

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Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of A-431 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and ...read more
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human colon shows nuclear membrane positivity in glandular and connective tissue cells.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of MCF7 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and ...read more
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of U-251 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and ...read more
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of HeLa cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and ...read more
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of U-2 OS cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red ...read more
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human oral mucosa shows nuclear membrane positivity in the epithelial cells.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human prostate shows nuclear membrane immunoreactivity in glandular epithelium cells.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human small intestine shows nuclear membrane immunoreactivity in glandular and connective tissue cells.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human testis shows strong nuclear membrane positivity in a subset of cells in the seminiferous tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL3929
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Lamin B1 Antibody (CL3929) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RTTRGKRKRVDVEESEASSSVSISHSASA
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LMNB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-59783 in the following applications:

Publications
Read Publications using
NBP2-59783 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Lamin B1 Antibody (CL3929)

  • ADLD
  • Lamin B1
  • lamin-B1
  • LMN
  • LMN2
  • LMNB
  • LMNB1
  • MGC111419

Background

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lamin B1 Antibody (NBP2-59783)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


Filter By Application
IF/IHC
(2)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-59783 Applications Species
Saleh, T;Alhesa, A;El-Sadoni, M;Abu Shahin, N;Alsharaiah, E;Al Shboul, S;Awad, H;Bloukh, S;Al-Balas, M;Alsalem, M;Azab, B;Aladily, TN; The Expression of the Senescence-Associated Biomarker Lamin B1 in Human Breast Cancer Diagnostics (Basel, Switzerland) [PMID: 35328162]
Saleh T, El-Sadoni M, Alhesa A Et al. Expression of Senescence and Apoptosis Biomarkers in Synchronous Bilateral Breast Cancer: A Case Report Current oncology (Toronto, Ont.) 2021-09-30 [PMID: 34677245] (IF/IHC, Human) IF/IHC Human
Molla S, Chatterjee S, Sethy C et al. Olaparib enhances curcumin-mediated apoptosis in oral cancer cells by inducing PARP trapping through modulation of BER and chromatin assembly DNA repair 2021-06-10 [PMID: 34144488]
Fang X, Dai E, Bai L et al. The HMGB1-AGER-STING1 pathway mediates the sterile inflammatory response to alkaliptosis Biochemical and biophysical research communications 2021-06-30 [PMID: 33992959]
Saleh T, Alhesa A, Al-Balas M et al. Expression of therapy-induced senescence markers in breast cancer samples upon incomplete response to neoadjuvant chemotherapy Bioscience reports 2021-05-28 [PMID: 33948615] (IF/IHC, Human) IF/IHC Human
Molla S, Hembram KC, Chatterjee S et al. PARP inhibitor Olaparib Enhances the Apoptotic Potentiality of Curcumin by Increasing the DNA Damage in Oral Cancer Cells through Inhibition of BER Cascade Pathol. Oncol. Res. 2019-11-25 [PMID: 31768967] (Human) Human
Hembram KC, Chatterjee S, Sethy C et al. Comparative and Mechanistic Study on the Anticancer Activity of Quinacrine-Based Silver and Gold Hybrid Nanoparticles in Head and Neck Cancer Mol. Pharm. 2019-07-01 [PMID: 31145852]

Review for Lamin B1 Antibody (NBP2-59783) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-59783:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ICC Human 11/07/2021
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Summary

ApplicationImmunocytochemistry
Sample Testedfibroblasts
SpeciesHuman
LotCL3929

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lamin B1 Antibody (NBP2-59783). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?
    • We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Verified Customer
11/07/2021
Application: ICC
Species: Human

Bioinformatics

Gene Symbol LMNB1