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Lamin A + C Antibody (133A2)

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Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688] - Staining of fiboblasts showing nuclear lamina
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688] - Staining of fiboblasts showing nuclear lamina
Immunohistochemistry-Paraffin: Lamin A + C Antibody (133A2) [NBP1-97688] - Analysis of human colon.
Immunohistochemistry-Frozen: Lamin A + C Antibody (133A2) [NBP1-97688] - Frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
133A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-97688AF350
Alexa Fluor 405
NBP1-97688AF405
Alexa Fluor 488
NBP1-97688AF488
Alexa Fluor 532
NBP1-97688AF532
Alexa Fluor 594
NBP1-97688AF594
Alexa Fluor 647
NBP1-97688AF647
Alexa Fluor 700
NBP1-97688AF700
Alexa Fluor 750
NBP1-97688AF750
Allophycocyanin
NBP1-97688APC
Biotin
NBP1-97688B
CoraFluor 1
NBP1-97688CL1
DyLight 350
NBP1-97688UV
DyLight 405
NBP1-97688V
DyLight 488
NBP1-97688G
DyLight 550
NBP1-97688R
DyLight 594
NBP1-97688DL594
DyLight 650
NBP1-97688C
DyLight 680
NBP1-97688FR
DyLight 755
NBP1-97688IR
FITC
NBP1-97688F
HRP
NBP1-97688H
Janelia Fluor 525
NBP1-97688JF525
Janelia Fluor 549
NBP1-97688JF549
Janelia Fluor 585
NBP1-97688JF585
Janelia Fluor 635
NBP1-97688JF635
Janelia Fluor 646
NBP1-97688JF646
Janelia Fluor 669
NBP1-97688JF669
PE
NBP1-97688PE
PerCP
NBP1-97688PCP
mFluor Violet 450 SE
NBP1-97688MFV450
mFluor Violet 500 SE
NBP1-97688MFV500
mFluor Violet 610 SE
NBP1-97688MFV610
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Lamin A + C Antibody (133A2) Summary

Description
The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Immunogen
Obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.
Marker
Nuclear Envelope Marker
Specificity
This antibody recognizes an epitope located between residues 598-611 of lamin A and therefore it reacts exclusively with lamin A.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
LMNA
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100 - 1:200
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:100 - 1:1000
Application Notes
This antibody is suitable for immunoblotting, immunocytochemistry on permeabilized cells, immunohistochemistry on frozen sections and paraffin embedded tissues, and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunocytochemistry, and immunohistochemistry with fluorescent secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

This antibody is CyTOF ready..

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS Since this antibody has to be purified from the culture medium by ion-exchange chromatography it will contain remnants of bovine serum albumin.
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for Lamin A + C Antibody (133A2)

  • CDCD1
  • CDDC
  • CMD1A
  • CMT2B1
  • dilated 1A (autosomal dominant)
  • EMD2
  • FPLD
  • HGPSFPL
  • lamin A/C
  • lamin A/C-like 1
  • lamin-A/C
  • LDP1
  • LFP
  • LGMD1B
  • limb girdle muscular dystrophy 1B (autosomal dominant)
  • LMN1IDC
  • LMNC
  • LMNL1
  • prelamin-A/C
  • PRO1,70 kDa lamin
  • progeria 1 (Hutchinson-Gilford type)
  • renal carcinoma antigen NY-REN-32

Background

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lamin A + C
Antibody (133A2)
NBP1-97688
Lamin A + C Antibody (133A2)
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol LMNA