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Lamin A + C Antibody (133A2) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
133A2
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-97688AF350
Alexa Fluor 405
NBP1-97688AF405
Alexa Fluor 488
NBP1-97688AF488
Alexa Fluor 532
NBP1-97688AF532
Alexa Fluor 594
NBP1-97688AF594
Alexa Fluor 647
NBP1-97688AF647
Alexa Fluor 700
NBP1-97688AF700
Alexa Fluor 750
NBP1-97688AF750
Allophycocyanin
NBP1-97688APC
Biotin
NBP1-97688B
DyLight 350
NBP1-97688UV
DyLight 405
NBP1-97688V
DyLight 488
NBP1-97688G
DyLight 550
NBP1-97688R
DyLight 594
NBP1-97688DL594
DyLight 650
NBP1-97688C
DyLight 680
NBP1-97688FR
DyLight 755
NBP1-97688IR
FITC
NBP1-97688F
HRP
NBP1-97688H
Janelia Fluor 525
NBP1-97688JF525
Janelia Fluor 549
NBP1-97688JF549
Janelia Fluor 585
NBP1-97688JF585
Janelia Fluor 635
NBP1-97688JF635
Janelia Fluor 646
NBP1-97688JF646
Janelia Fluor 669
NBP1-97688JF669
PE
NBP1-97688PE
PerCP
NBP1-97688PCP
Unconjugated
NBP1-97688
mFluor Violet 450 SE
NBP1-97688MFV450
mFluor Violet 500 SE
NBP1-97688MFV500
mFluor Violet 610 SE
NBP1-97688MFV610
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Lamin A + C Antibody (133A2) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.
Marker
Nuclear Envelope Marker
Specificity
This antibody recognizes an epitope located between residues 598-611 of lamin A and therefore it reacts exclusively with lamin A.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
LMNA
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined..

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Ion exchange chromatography

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Lamin A + C Antibody (133A2) [CoraFluor™ 1]

  • CDCD1
  • CDDC
  • CMD1A
  • CMT2B1
  • dilated 1A (autosomal dominant)
  • EMD2
  • FPLD
  • HGPSFPL
  • lamin A/C
  • lamin A/C-like 1
  • lamin-A/C
  • LDP1
  • LFP
  • LGMD1B
  • limb girdle muscular dystrophy 1B (autosomal dominant)
  • LMN1IDC
  • LMNC
  • LMNL1
  • prelamin-A/C
  • PRO1,70 kDa lamin
  • progeria 1 (Hutchinson-Gilford type)
  • renal carcinoma antigen NY-REN-32

Background

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lamin A + C
Antibody (133A2) [Cor ...
NBP1-97688CL1
Lamin A + C Antibody (133A2) [CoraFluor™ 1]
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol LMNA