![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lactate Dehydrogenase A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lactate Dehydrogenase A.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2D11-Western-Blot-NBP1-47822-img0015.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lactate Dehydrogenase A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lactate Dehydrogenase A.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | OTI2D11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell. |
| Specificity | This antibody is specific for Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LDHA |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB, ICC/IF reactivity reported in (PMID: 22659615). |
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| Theoretical MW | 36.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Lactate Dehydrogenase A/LDHA Antibody (NBP1-47822)Find related products by research area.
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![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2D11-Immunohistochemistry-Paraffin-NBP1-47822-img0014.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2D11-Immunohistochemistry-Paraffin-NBP1-47822-img0009.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2D11-Immunohistochemistry-Paraffin-NBP1-47822-img0010.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2D11-Immunohistochemistry-Paraffin-NBP1-47822-img0011.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2D11-Immunohistochemistry-Paraffin-NBP1-47822-img0012.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2D11-Immunohistochemistry-Paraffin-NBP1-47822-img0013.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.")
![Immunoprecipitation: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Lane 1: Negative Control Lane 2: NBP1-47824 Lane 3: NBP1-47825 Lane 4: NBP1-47823 Lane 5: NBP1-47822](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2D11-Immunoprecipitation-NBP1-47822-img0016.jpg "Immunoprecipitation: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Lane 1: Negative Control Lane 2: NBP1-47824 Lane 3: NBP1-47825 Lane 4: NBP1-47823 Lane 5: NBP1-47822")
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)](http://images.novusbio.com/fullsize/nbp1-47822_mouse-lactate-dehydrogenase-a-ldha-mab-oti2d11-17720241003223.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")