![Western Blot: Ku70/XRCC6 Antibody [NBP2-19311] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Western-Blot-NBP2-19311-img0007.jpg "Western Blot: Ku70/XRCC6 Antibody [NBP2-19311] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Func, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Ku70/XRCC6. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | XRCC6 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Ku70/XRCC6 Antibody (NBP2-19311)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody [NBP2-19311] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku70 protein stained by Ku70 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19311-img0005.jpg "Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody [NBP2-19311] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku70 protein stained by Ku70 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-19311] - Human cervical cancer. Ku70 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-19311-img0006.jpg "Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-19311] - Human cervical cancer. Ku70 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Ku70 Antibody [NBP2-19311] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Ku70-Antibody-Immunohistochemistry-Paraffin-NBP2-19311-img0002.jpg "Immunohistochemistry-Paraffin: Ku70 Antibody [NBP2-19311] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.")
![Immunoprecipitation: Ku70 Antibody [NBP2-19311] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2. 5 ug of Ku70 antibody 7. 5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Ku70-Antibody-Immunoprecipitation-NBP2-19311-img0004.jpg "Immunoprecipitation: Ku70 Antibody [NBP2-19311] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2. 5 ug of Ku70 antibody 7. 5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")