![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0010.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 17 amino acids near the center of human Klotho. The immunogen is located within amino acids 410-460 of Klotho. Amino Acid Sequence: SGTTKRRDDAKYMYYLK |
| Specificity | Three isoforms of Klotho are known to exist. Human Klotho has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD). Mouse Klotho has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD). Rat Klotho has one isoform (1014aa, 117kD). NBP1-76511 can detect human, mouse and rat. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KL |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 116 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Klotho Antibody (NBP1-76511)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 20 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-76511-img0002.jpg "Immunocytochemistry/Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 20 ug/ml.")
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Endothelial klotho staining. Image collected and cropped by CiteAb from the following publication (cardiab.biomedcentral.com/articles/10.1186/s12933-016-0414-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-NBP1-76511-img0008.jpg "Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Endothelial klotho staining. Image collected and cropped by CiteAb from the following publication (cardiab.biomedcentral.com/articles/10.1186/s12933-016-0414-5), licensed under a CC-BY license.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - ARPE-19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin-3a, hypoxia + AAV-p53 mutant infection and hypoxia + miRNA-34a inhibitor. Western blot assay of Klotho in ARPE-19 cells was performed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Klotho Antibody - BSA Free-Western Blot-NBP1-76511-img0011.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - ARPE-19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin-3a, hypoxia + AAV-p53 mutant infection and hypoxia + miRNA-34a inhibitor. Western blot assay of Klotho in ARPE-19 cells was performed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362/) licensed under a CC-BY license.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Validation in Rat Kidney Tissue LysateLoading: 15ug of lysates per lane.Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0007.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Validation in Rat Kidney Tissue LysateLoading: 15ug of lysates per lane.Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KLOTHO NBP1-76511 (2 ug/mL), KLOTHO, competitor antibody (4 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0009.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KLOTHO NBP1-76511 (2 ug/mL), KLOTHO, competitor antibody (4 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-76511-img0003.jpg "Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.")
![Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Staining of rat heart tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-76511-img0004.jpg "Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Staining of rat heart tissue with antibody at 2.5 ug/ml.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Blockade of the HIF‐1 alpha /p53/miRNA‐34a/Klotho axis decreases the leakage & area of mouse laser‐induced CNV. The mice were divided into the following groups: normal, CNV 7 d, CNV 7 d + 0.1% DMSO, CNV 7 d + digoxin (oral; 2 mg/kg for 7 d), CNV 7 d + AAV‐p53 mutant (intravitreal injection; approximately 3 μL, 3 × 1010 viral particles/mL), CNV 7 d + miRNA‐34a inhibitor (intravitreal injection; 1 μg) & CNV 7 d + AAV‐Klotho full‐length plasmid (intravitreal injection, 2 μL, 5 × 1010 viral particles/mL). A, Western blot was performed to measure HIF‐1 alpha , p‐p53 (S15), p53 (S20), p‐p53 (S46), p53 & Klotho protein levels. B, The relative protein levels of HIF‐1 alpha /GAPDH (B), p‐p53 (S15)/p53 (C), p‐p53 (S20)/p53 (D), p‐p53 (S46)/p53 (E), p53/GAPDH (F) & Klotho/GAPDH (G) were analysed. ***P Figure 4 Immunohistochemistry Validation of Klotho in Mouse Heart Tissue Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202419353832.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Blockade of the HIF‐1 alpha /p53/miRNA‐34a/Klotho axis decreases the leakage & area of mouse laser‐induced CNV. The mice were divided into the following groups: normal, CNV 7 d, CNV 7 d + 0.1% DMSO, CNV 7 d + digoxin (oral; 2 mg/kg for 7 d), CNV 7 d + AAV‐p53 mutant (intravitreal injection; approximately 3 μL, 3 × 1010 viral particles/mL), CNV 7 d + miRNA‐34a inhibitor (intravitreal injection; 1 μg) & CNV 7 d + AAV‐Klotho full‐length plasmid (intravitreal injection, 2 μL, 5 × 1010 viral particles/mL). A, Western blot was performed to measure HIF‐1 alpha , p‐p53 (S15), p53 (S20), p‐p53 (S46), p53 & Klotho protein levels. B, The relative protein levels of HIF‐1 alpha /GAPDH (B), p‐p53 (S15)/p53 (C), p‐p53 (S20)/p53 (D), p‐p53 (S46)/p53 (E), p53/GAPDH (F) & Klotho/GAPDH (G) were analysed. ***P Figure 4 Immunohistochemistry Validation of Klotho in Mouse Heart Tissue Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 4 Immunohistochemistry Validation of Klotho in Mouse Heart Tissue Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202419562112.jpg "Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 4 Immunohistochemistry Validation of Klotho in Mouse Heart Tissue Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202419353822.jpg "Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).")
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart Tissue Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202420272449.gif "Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart Tissue Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart Tissue Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-271120242013162.gif "Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart Tissue Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202420212384.jpg "Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")