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KLC2 Antibody

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Genetic Strategies: Western Blot: KLC2 Antibody [NBP1-83723] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: KLC2 Antibody [NBP1-83723] - Staining of human cell line A-431 shows positivity in plasma membrane and cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: KLC2 Antibody [NBP1-83723] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Independent Antibodies: Western Blot: KLC2 Antibody [NBP1-83723] - Analysis using Anti-KLC2 antibody NBP1-83723 (A) shows similar pattern to independent antibody NBP1-83722 (B).
Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human colon.
Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human testis.
Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human lymph node.
Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human cerebral cortex shows moderate positivity in neuropil.
Independent Antibodies: Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human cerebral cortex, fallopian tube, prostate and testis using Anti-KLC2 antibody NBP1-83723 (A) shows similar ...read more
Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human Fallopian tube shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: KLC2 Antibody [NBP1-83723] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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KLC2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCILLRRSLE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KLC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
KLC2 Protein (NBP1-83723PEP)
Publications
Read Publication using NBP1-83723.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for KLC2 Antibody

  • FLJ12387
  • kinesin light chain 2
  • KLC 2

Background

Kinesin is a molecular motor that generates ATP-dependent movement of vesicles and organelles along microtubules. Kinesin consists of 2 light chains, such as KLC2, and 2 heavy chains (see KIF5B; MIM 602809) in a 1:1 stoichiometric ratio (Rahman et al., 1998 [PubMed 9624122]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLC2 Antibody (NBP1-83723)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KLC2 Antibody (NBP1-83723) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KLC2