![Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Western-Blot-NBP2-22321-img0010.jpg "Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Western blot image submitted by a verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 425 to 475 of human Golgin B1 using the numbering given in entry NP_004478.3 (GeneID 2804). |
| Marker | Golgi Apparatus Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GOLGB1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Immunohistochemistry_6703.jpg)
![Western Blot ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Flow_Cytometry_20028.jpg)
![Simple Western ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Simple_Western_20109.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Armen Petrosyan |
IF | Human and PC-3 cells | 08/19/2020 |
Summary
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Enlarge |
reviewed by:
Armen Petrosyan |
WB | Human | 11/20/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for GOLGB1/Giantin Antibody (NBP2-22321)Find related products by research area.
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| Armen Petrosyan 08/19/2020 |
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| Application: | IF | |
| Species: | Human and PC-3 cells |
| Armen Petrosyan 11/20/2018 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | GOLGB1 |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - PC-3 human prostate cancer cell line. Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 micron. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22321-img0011.jpg "Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - PC-3 human prostate cancer cell line. Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 micron. ICC/IF image submitted by a verified customer review.")
![Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Western-Blot-NBP2-22321-img0004.jpg "Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Untreated MDA-MB-231 cells showed perinuclear colonization of Giantin and other proteins, demonstrating this cell line exhibits the architecture of a typical Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. Secondary antibodies in other channels. Nuclei were stained with DAPI (gray channel). Fluorescence microscopy was used to examine stained cells. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0005.jpg "Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Untreated MDA-MB-231 cells showed perinuclear colonization of Giantin and other proteins, demonstrating this cell line exhibits the architecture of a typical Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. Secondary antibodies in other channels. Nuclei were stained with DAPI (gray channel). Fluorescence microscopy was used to examine stained cells. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Effect of ActD on the perinuclear localization of the Golgi apparatus of MDA-MB-231 cells assessed by immunofluorescence to Giantin, GM130 and TGN46. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0009.jpg "Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Effect of ActD on the perinuclear localization of the Golgi apparatus of MDA-MB-231 cells assessed by immunofluorescence to Giantin, GM130 and TGN46. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Shows the combination effects of antitumor drugs and ARF1 disruptors on the MDA-MB-231 cells Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0008.jpg "Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Shows the combination effects of antitumor drugs and ARF1 disruptors on the MDA-MB-231 cells Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.")
![Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunoprecipitation-NBP2-22321-img0003.jpg "Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
