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JunD Antibody

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse JunD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5526) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for JunD at approximately 40 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and MCF-7 human breast cancer cell line. PVDF ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5526-SP
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JunD Antibody Summary

Immunogen
E. coli-derived recombinant human JunD
Lys47-Gly156
Accession # P17535
Specificity
Detects human and mouse JunD in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
JUND
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JunD Antibody

  • activator protein 1
  • AP-1
  • jun D proto-oncogene
  • JunD
  • JunD-FL isoform
  • transcription factor jun-D

Background

JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or FOS family members to form the AP1 transcription factor. Unlike c-Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD has two isoforms full length at 347 amino acids (aa), the other 303 aa which initiates translation at aa 44. Over the sequence used as an immunogen, human JunD shares 80% aa identity with mouse and rat JunD. The activation of JunD appears to reduce the proliferation of breast cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol JUND
Uniprot