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JIK Antibody

Images

 
Western Blot: JIK Antibody [NBP1-84988] - Analysis in control (vector only transfected HEK293T lysate) and TAOK3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: JIK Antibody [NBP1-84988] - Staining of human stomach shows strong cytoplasmic positivity in Parietal cells.
Orthogonal Strategies: Western Blot: JIK Antibody [NBP1-84988] - Analysis in human cell lines SK-MEL-30 and U2OS using Anti-TAOK3 antibody. Corresponding TAOK3 RNA-seq data are presented for the same cell lines. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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JIK Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ESQEDEEDSEHGTSLNREMDSLGSNHSIPSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGDPRPEPRPTQSVQSQALHYRNR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAOK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
JIK Protein (NBP1-84988PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for JIK Antibody

  • CTCL-associated antigen HD-CL-09
  • Cutaneous T-cell lymphoma-associated antigen HD-CL-09
  • Dendritic cell-derived protein kinase
  • DPKJNK/SAPK-inhibitory kinase
  • EC 2.7.11
  • FLJ31808
  • hKFC-A
  • JIKEC 2.7.11.1
  • Jun kinase-inhibitory kinase
  • KDS
  • Kinase from chicken homolog A
  • MAP3K18DKFZp666H245
  • serine/threonine-protein kinase TAO3
  • STE20-like kinase
  • TAO kinase 3
  • Thousand and one amino acid protein 3

Background

JIK/TAOK3 inhibits the basal activity of Jun kinase. It is negatively regulated by epidermal growth factor (EGF). When it is overexpressed, it may activate ERK1/ERK2 and JNK/SAPK. It may also play a role in controlling the expression level of BimEL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TAOK3